ابداع روشی کم‌هزینه و دقیق برای خالص‌سازی فاکتور رشد اپیدرمی انسانی نوترکیب (hEGF)

[1] Sharma K., Cherish Babu P.V., Sasidhar  P., Srinivas V.K., Krishna Mohan V., Krishna Ella, Recombinant Human Epidermal Growth Factor Inclusion Body Solubilization and Refolding at Large Scale using Expanded-Bed Adsorption Chromatography from Escherichia coli, Protein Expression and Purification, 60: 7-14 (2008).

[2]  Huang R.C., Lam E., Chen Y.H., Hackett J, Lam T.L., Liu D., Ma M.C., Siu K.L., Sivakesava S., Xu Z.N., Wong R.S.C. Wong W.K.R., Human Epidermal Growth Factor Excreted by Recombinant Escherichia coli K-12 has the Correct N-Terminus and is Fully Bioactive, Process Biochem., 35: 1-5 (1999).

[3] شجاع‌الساداتی، سید عباس؛ خلیل‌زاده، رسول؛ محمدیان، جعفر؛ "کلونینگ و بیان فاکتور رشد اپیدرمی انسانی در باکتری اشرشیاکلی"، گزارش پروژه تحقیقاتی، صفحات2-20 (1386).

[4] Heo J.H., Won H.S., Kang H.A., Rhee S.K. Chung B.H., Purification of Recombinant Human Epidermal Growth Factor Secreted from the Methylotrophic Yeast Hansenula Polymeorpha, Protein Expression and Purification, 24: 117-122 (2002).

[5] Soler L.F., Cedano J., Querol E. Liorens R., Cloning, Expression and Purification of Human Epidermal Growth Factor using Different Expression Systems, Journal of Chromatography, 788: 113-123 (2003).

[6] Finkenaur, “Stabilized Compositions Containing Epidermal Growth Factor”, US Patent References, Publication Number EP 0267015 A2 (1988).

[7] Mount C.D., Lukas T.J., Orth D.N., Purification and Characterization of Epidermal Growth Factor and Epidermal Growth Factor Fragments from Large volumes of Human Urine, Arch Biochem. Biophys., 240: 33-42 (1985).

[8] Lee J.Y., Chang S.Y., Chung Y., Lee Y.S. Lee E.K., Scale-up Process for Expression and Renaturation of Recombinant Human Epidermal Growth Factor from Escherichia coli Inclusion Bodies, Biotechnol. Appl. Biochem., 31: 245-248 (2000).

[9] Lee Y.S., Suh C.W., Park S.K. Lee E.K., Purification of Soluble Human Epidermal Growth Factor (hEGF) from Recombinant Escherichia coli Culture Broth by Using Expanded-Bed Adsorption Chromatography, Biotechnol. Appl. Biochem., 38: 9-13 (2003).

[10] Abdull Razis A.F., Ismail E.N., Hambali Z., Abdullah M.N.H., Ali A.M. Mohd Lila M.A.,The Periplasmic Expression of Recombinant Human Epidermal Growth Factor (hEGF) in Escherichia coli, Asia Pacific Journal of Molecular Biology and Biotechnology, 14: 41-45 (2006).

[11] اسفندیار، سمانه؛ "خالص سازی اینترلوکین-2 تولید شده در باکتری اشرشیاکلی نوترکیب"، پایان‌نامه کارشناسی ارشد، دانشگاه تربیت ­مدرس (1387).

[12] Getz E.B., Xiao M., Chakrabarty T., Cooke R. Selvin P.R., A Comparison between the Sulfhydryl Reductants Tris(2-carboxyethyl)phosphine and Dithiothreitolfor Use in Protein Biochemistry, Analytical Biochem., 273: 73-80 (1999).