نشریه شیمی و مهندسی شیمی ایران

نشریه شیمی و مهندسی شیمی ایران

بهینه‌سازی نانوکامپوزیت­ های مس­اکسید-زئولیت/گرافن اکسید برای رهایش کنترل‌شده دوکسوروبیسین جهت مقابله با سلول‌های سرطان پستان HS-578T

نوع مقاله : علمی-پژوهشی

نویسندگان
سحر مهاجری 1
هاشم یعقوبی 2
1 گروه شیمی، دانشگاه آزاد اسلامی، واحد اردبیل، اردبیل، ایران
2 گروه زیست شناسی‌، دانشگاه آزاد اسلامی، واحد اردبیل، اردبیل، ایران
چکیده
در این پژوهش نانوکامپوزیت­ های زئولیت–مس­اکسید/گرافن­اکسید به‌عنوان حامل داروی دوکسوروبیسین (DOX) سنتز و مشخصه‌یابی شدند. برای بررسی ریخت­ شناسی، بلوری و گروه‌های عاملی از روش­ های شناسایی نظیر SEM، TEM، XRD و FTIR استفاده گردید. طبق نتایج حاصل از آنالیز XRD و روش ویلیامسون-هال، اندازه بلورک‌های مس‌اکسید به‌طور مجزا ۱۵-۳۰ نانومتر و اندازه گرافن‌اکسید (GO) ۱-۵ نانومتر بود. در نانوکامپوزیت سه‌جزئی (زئولیت/مس‌اکسید/گرافن‌اکسید)، اندازه بلورک‌های مس‌اکسید (CuO) به‌طور چشمگیری به ۳-۱۵ نانومتر کاهش یافت، که نشان‌دهنده اثر مهارکنندگی قوی بسترهای مرکب بر رشد نانوذرات و ایجاد ساختار مناسب برای رهایش کنترل‌شده دارو است. بارگذاری دارو بر اساس ظرفیت جذب تعیین و الگوی رهایش در شرایط 4/7pH= (فیزیولوژیک) و ۵/۵pH= (اسیدی) طی 72 ساعت ارزیابی شد. بر اساس نتایج حاصل نانوکامپوزیت زئولیت-مس­اکسید/گرافن­اکسید رهایش کنترل‌شده‌تری از داروی DOX در 4/7pH= نشان داد، اما در محیط اسیدی (۵/۵pH=) رهایش انفجاری و افزایش‌یافته‌ای داشت. بر اساس نتایج حاصل از سمیت سلولی، ترکیبات بدون دارو (زئولیت، نانوکامپوزیت‌ها و گرافن­اکسید) سمیت بسیار پایینی بر روی سلول‌های HS-578T داشتند (IC50 >100 µg/ml) ، اما پس از بارگذاری داروی DOX، سمیت همه نمونه‌ها به‌طور چشمگیری افزایش یافت، به‌طوری­که نانوکامپوزیت زئولیت-مس­اکسید/گرافن­اکسید حاوی DOX با کمترین مقدار IC50 (µg/ml 12/37)، بیشترین سمیت را جهت مهار رشد سلول‌های سرطانی داشتند. آزمون آپوپتوز نشان داد نانوکامپوزیت دارویی مرگ برنامه‌ریزی‌شده را به‌طور معنی‌دار القا می‌کند. این نتایج کارایی بالای نانوکامپوزیت را در دارورسانی هدفمند و کاهش عوارض جانبی تأیید می‌نماید.
کلیدواژه‌ها
رده سلول سرطانی HS-578T
دوکسوروبیسین
زئولیت
گرافن­ اکسید
نانو­کامپوزیت
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