Nashrieh Shimi va Mohandesi Shimi Iran

Nashrieh Shimi va Mohandesi Shimi Iran

Design and Synthesis of Efficient New Nanocomposites Fe3O4@MCM-41/HAP/APTES and CMC/MMT/HAP for Controlled Release Drug Delivery: Targeted Delivery of Teriparatide in Bone Tissue Engineering

Document Type : Research Article

Authors
Hamid Reza Hosseini 1
Majid Abdouss 2
Mostafa Golshekan 3
Pedram Tehrani 1
1 Department of Biomedical Engineering, Central Tehran Branch, Islamic Azad University, Tehran, IR. IRAN
2 Department of Chemistry, Amirkabir University of Technology. Tehran, I.R. IRAN
3 Guilan Road Trauma Research Center, Guilan University of Medical Sciences Rasht. I.R. IRAN
Abstract
The drug delivery approach in bone tissue engineering faces unique challenges due to the complex anatomy of bone and the limitations of drug delivery. In response, new pH-responsive nanocomposites, including magnetite nanoparticles (Fe3O4), Mobil Composition No. 41 (MCM-41), hydroxyapatite (HAP), 3-aminopropyltriethoxysilane (APTES), carboxymethyl cellulose (CMC), and montmorillonite (MMT) [(Fe3O4@MCM-41/HAP/APTES)] and hydrogel [(CMC/MMT/HAP)], have been developed for accurate delivery of teriparatide (PTH (1-34)) with the aim of increasing solubility, drug stability, and controlled release. These nanocomposites provide an efficient injectable dosing regimen that eliminates the side effects, safety concerns, and discomfort associated with repeated injections. Nanocomposites were characterized by various analytical techniques, including X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FT-IR), zeta potential analysis, dynamic light scattering (DLS), and field emission scanning electron microscopy (FE-SEM). The average crystal diameter of the nanocomposites was calculated as 27.6 ± 4 and 29.2 ± 1.4 nm, respectively, from Scherer's equation, and the drug-loaded nanocarriers had a hydrodynamic diameter of 417.023 ± 8.3 and 193.48 ± 3.8 nm, respectively, and a stable surface charge of -31 and -40 mV, respectively. In addition, the efficiency of loading and entrapment was determined to be 37% and 90% for the first nanocomposite and 38% and 82% for the second nanocomposite, respectively. Drug release experiments using the dialysis method combined with high-performance liquid chromatography (HPLC) analysis showed a sustained release pattern. As pH decreased from 7.4 to 5.6, there was a corresponding increase in drug release. The results of the study of drug release kinetics followed the Higuchi model, which fully corresponded to the characteristics of the release target of the nanocarriers and teriparatide drug, and confirmed the preservation of the integrity of the nanocarrier. The results of the cytotoxicity test within 24 hours on the NIH3T3 cell line not only showed no toxicity but also recorded the effect of cell proliferation and differentiation, which was controlled and limited in the case of the Saos-2 cell line. Based on these findings, nanocomposites designed and manufactured with the greatest impact of components in bone tissue regeneration, as a drug delivery system, provide a very efficient approach to increase therapeutic effectiveness and thus overcome the limitations associated with drug delivery.
Keywords
Keyword: Drug delivery nanocarriers
Hydroxyapatite
Magnetite nanoparticles
Montmorillonite
carboxymethyl cellulose
Teriparatide

Subjects

[1] Patel D., Wairkar S., Bone Regeneration in Osteoporosis: Opportunities and Challenges, Drug Delivery and Translational Research, 13(2): 419-432 (2023).
[2] Somersalo A., Paloneva J., Kautiainen H., LÖNnroos E., HeinÄNen M., Kiviranta I., Increased Mortality After Lower Extremity Fractures in Patients <65 Years of Age, Acta Orthopaedica, 87(6): 622-625 (2016).
[3] Riaz S., Shakil Ur Rehman S., Hafeez S., Hassan D., Effects of Kinect-Based Virtual Reality Training on Bone Mineral Density and Fracture Risk in Postmenopausal Women with Osteopenia: A Randomized Controlled Trial, Scientific Reports, 14(1): 6650 (2024).
[4] Albrecht B.M., Stalling I., Foettinger L., Recke C., Bammann K., Adherence to Lifestyle Recommendations for Bone Health in Older Adults with and without Osteoporosis: Cross-Sectional Results of the OUTDOOR ACTIVE Study, Nutrients, 14(12): 2463 (2022).
[5] Jones M.S., Waterson B., Principles of Management of Long Bone Fractures and Fracture Healing, Surgery (Oxford), 38(2): 91-99 (2020).
[6] Chen J., Ashames A., Buabeid M.A., Fahelelbom K.M., Ijaz M., Murtaza G., Nanocomposites Drug Delivery Systems for the Healing of Bone Fractures, International Journal of Pharmaceutics, 585: 119477 (2020).
[7] Cometa S., Bonifacio M.A., Tranquillo E., Gloria A., Domingos M., De Giglio E., A 3D Printed Composite Scaffold Loaded with Clodronate to Regenerate Osteoporotic Bone: In Vitro Characterization, Polymers, 13(1): 150 (2021).
[8] Russo T., De Santis R., Peluso V., Gloria A., Multifunctional Bioactive Magnetic Scaffolds with Tailored Features for Bone Tissue Engineering, Magnetic Nanoparticles in Human Health and Medicine, 87-112 (2021).
[9] Iranmanesh P., Ehsani A., Khademi A., Asefnejad A., Shahriari S., Soleimani M., Ghadiri Nejad M., Saber-Samandari S., Khandan A., Application of 3D Bioprinters for Dental Pulp Regeneration and Tissue Engineering (Porous Architecture), Transport in Porous Media, 142(1): 265-293 (2022).
[10] Karimi M., Asefnejad A., Aflaki D., Surendar A., Baharifar H., Saber-Samandari S., Khandan A., Khan A., Toghraie D., Fabrication of Shapeless Scaffolds Reinforced with Baghdadite-Magnetite Nanoparticles Using a 3D Printer and Freeze-Drying Technique, Journal of Materials Research and Technology, 14: 3070-3079 (2021).
[11] Pavlychuk T., Chernogorskyi D., Chepurnyi Y., Neff A., Kopchak A., Application Of CAD/CAM Technology For Surgical Treatment Of Condylar Head Fractures: A Preliminary Study, Journal of Oral Biology and Craniofacial Research, 10(4): 608-614 (2020).
[12] Momesso G.A.C., Polo T.O.B., da Silva W.P.P., Barbosa S., Freitas G.P., Lopes H.B., Rosa A.L., Cordeiro J.M., Toro L.F., Chiba F.Y., Matsushita D.H., Louzada M.J.Q., da Cruz N.C., Barão V.A.R., Faverani L.P., Miniplates Coated by Plasma Electrolytic Oxidation Improve Bone Healing of Simulated Femoral Fractures on Low Bone Mineral Density Rats, Materials Science and Engineering, C, 120: 111775 (2021).
[13] Al-Hourani K., Tsang S.-T.J., Simpson A.H.R.W., Osteoporosis: Current Screening Methods, Novel Techniques, and Preoperative Assessment of Bone Mineral Density, Bone & Joint Research, 10(12): 840-843 (2021).
[14] Kumar R., Sarkar C., Panja S., Khatua C., Gugulothu K., Sil D., Biomimetic Nanocomposites for Biomedical Applications, »Biorenewable Nanocomposite Materials, Vol. 1: Electrocatalysts and Energy Storage«, American Chemical Society, 163-196 (2022).
[15] Kim T., See C.W., Li X., Zhu D., Orthopedic Implants and Devices for Bone Fractures and Defects: Past, Present and Perspective, Engineered Regeneration, 1: 6-18 (2020).
[16] Bahari Javan N., Rezaie Shirmard L., Jafary Omid N., Akbari Javar H., Rafiee Tehrani M., Abedin Dorkoosh F., Preparation, Statistical Optimisation and in Vitro Characterisation of Poly (3-Hydroxybutyrate-Co-3-Hydroxyvalerate)/Poly (Lactic-Co-Glycolic Acid) Blend Nanoparticles for Prolonged Delivery of Teriparatide, Journal of Microencapsulation, 33(5): 460-474 (2016).
[17] Verma R., Mishra S.R., Gadore V., Ahmaruzzaman M., Hydroxyapatite-Based Composites: Excellent Materials for Environmental Remediation and Biomedical Applications, Advances in Colloid and Interface Science, 315: 102890 (2023).
[18] Radulescu D.-E., Vasile O.R., Andronescu E., Ficai A., Latest Research of Doped Hydroxyapatite for Bone Tissue Engineering, International Journal of Molecular Sciences, 24(17): 13157 (2023).
[19] Mondal S.K., Dorozhkin S.V., Pal U., Recent Progress on Fabrication and Drug Delivery Applications of Nanostructured Hydroxyapatite, Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology, 10(4): e1504 (2018).
[20] Oni O.P., Hu Y., Tang S., Yan H., Zeng H., Wang H., Ma L., Yang C., Ran J., Syntheses and Applications of Mesoporous Hydroxyapatite: A Review, Materials Chemistry Frontiers, 7(1): 9-43 (2023).
[21] Habraken W., Habibovic P., Epple M., Bohner M., Calcium Phosphates in Biomedical Applications: Materials for the Future?, Materials Today, 19(2): 69-87 (2016).
[22] Hosseini S.M., Abdouss M., Mazinani S., Soltanabadi A., Kalaee M., Modified Nanofiber Containing Chitosan and Graphene Oxide-Magnetite Nanoparticles as Effective Materials for Smart Wound Dressing, Composites Part B: Engineering 231: 109557 (2022).
[23] Shuai C., Yang W., He C., Peng S., Gao C., Yang Y., Qi F., Feng P., A Magnetic Micro-Environment in Scaffolds for Stimulating Bone Regeneration, Materials & Design, 185:  108275 (2020).
[24] Khalid A., Ahmed R.M., Taha M., Soliman T.S., Fe3O4 Nanoparticles and Fe3O4 @SiO2 Core-Shell: Synthesize, Structural, Morphological, Linear, and Nonlinear Optical Properties, Journal of Alloys and Compounds, 947: 169639 (2023).
[25] Marycz K., Sobierajska P., Wiglusz R., Idczak R., Nedelec J.-M., Fal A., Kornicka-Garbowska K., Fe3O4Magnetic Nanoparticles Under Static Magnetic Field Improve Osteogenesis via RUNX-2 and Inhibit Osteoclastogenesis by the Induction of Apoptosis, Int J Nanomedicine 15: 10127-10148 (2020).
[26] Ulu A., Noma S.A.A., Koytepe S., Ates B., Magnetic Fe3O4@MCM-41 Core–Shell Nanoparticles Functionalized with Thiol Silane for Efficient L-Asparaginase Immobilization, Artificial Cells, Nanomedicine, and Biotechnology, 46(sup2): 1035-1045 (2018).
[27] Shadabfar M., Abdouss M., Khonakdar H.A., Synthesis, Characterization, and Evaluation of a Magnetic Molecular Imprinted Polymer for 5-Fluorouracil as an Intelligent Drug Delivery System for Breast Cancer Treatment, Journal of Materials Science, 55(26): 12287-12304 (2020).
[28] Pourhasan-Kisomi R., Shirini F., Golshekan M., Introduction of Organic/Inorganic Fe3O4@MCM-41@Zr-Piperazine Magnetite Nanocatalyst for the Promotion of the Synthesis of Tetrahydro-4H-Chromene and Pyrano[2,3-d]Pyrimidinone Derivatives, Applied Organometallic Chemistry, 32(7): e4371 (2018).
[29] Venedicto M., Carrier J., Na H., Chang C.-Y., Radu D.R., Lai C.-Y., Disulfide-Modified Mesoporous Silica Nanoparticles for Biomedical Applications, Crystals, 13(7): 1067 (2023).
[30] Babaki M., Yousefi M., Habibi Z., Brask J., Mohammadi M., Preparation of Highly Reusable Biocatalysts by Immobilization of Lipases on Epoxy-Functionalized Silica for Production of Biodiesel from Canola Oil, Biochemical Engineering Journal, 101: 23-31 (2015).
[31] Jafarzadeh A., Sohrabnezhad S., Zanjanchi M.A., Arvand M., Synthesis and Characterization of Thiol-Functionalized MCM-41 Nanofibers and Its Application as Photocatalyst, Microporous and Mesoporous Materials, 236: 109-119 (2016).
[32] Nayl A.A., Abd-Elhamid A.I., Aly A.A., Bräse S., Recent Progress in the Applications of Silica-Based Nanoparticles, RSC Advances, 12(22): 13706-13726 (2022).
[33] Popova T., Tzankov B., Voycheva C., Spassova I., Kovacheva D., Tzankov S., Aluani D., Tzankova V., Lambov N., Mesoporous Silica MCM-41 and HMS as Advanced Drug Delivery Carriers for Bicalutamide, Journal of Drug Delivery Science and Technology, 62: 102340 (2021).
[34] Trzeciak K., Kaźmierski S., Wielgus E., Potrzebowski M.J., DiSupLo - New Extremely Easy and Efficient Method for Loading of Active Pharmaceutical Ingredients Into the Pores of MCM-41 Mesoporous Silica Particles, Microporous and Mesoporous Materials, 308: 110506 (2020).
[35] Szewczyk A., Skwira A., Konopacka A., Sądej R., Prokopowicz M., Mesoporous Silica-Bioglass Composite Pellets as Bone Drug Delivery System with Mineralization Potential, International Journal of Molecular Sciences, 22(9): 4708 (2021).
[36] Asadi E., Abdouss M., Leblanc R.M., Ezzati N., Wilson J.N., Kordestani D., Synthesis, Characterization and in Vivo Drug Delivery Study of a Biodegradable Nano-Structured Molecularly Imprinted Polymer Based on Cross-Linker of Fructose, Polymer, 97: 226-237 (2016).
[37] Esmaeilnejad-Ahranjani P., Kazemeini M., Singh G., Arpanaei A., Amine-Functionalized Magnetic Nanocomposite Particles for Efficient Immobilization of Lipase: Effects of Functional Molecule Size on Properties of the Immobilized Lipase, RSC Advances, 5(42): 33313-33327 (2015).
[38] Popescu S., Ardelean I.L., Gudovan D., Rădulescu M., Ficai D., Ficai A., Vasile B.Ş., Andronescu E., Multifunctional Materials Such as MCM-41/Fe3O4/Folic Acid as Drug Delivery System, Rom J Morphol Embryol, 57(2): 483-489 (2016).
[39] Rao Z., Ge H., Liu L., Zhu C., Min L., Liu M., Fan L., Li D., Carboxymethyl Cellulose Modified Graphene Oxide as pH-Sensitive Drug Delivery System, International Journal of Biological Macromolecules, 107: 1184-1192 (2018).
[40] Sandomierski M., Buchwald Z., Voelkel A., Calcium Montmorillonite and Montmorillonite with Hydroxyapatite Layer As Fillers in Dental Composites with Remineralizing Potential, Applied Clay Science, 198: 105822 (2020).
[41] Li D., Li P., Xu Y., Guo W., Li M., Chen M., Wang H., Lin H., Progress in Montmorillonite Functionalized Artificial Bone Scaffolds: Intercalation and Interlocking, Nanoenhancement, and Controlled Drug Release, Journal of Nanomaterials, 2022: 7900382 (2022).
[42] Hong H.-J., Kim J., Kim D.-Y., Kang I., Kang H.K., Ryu B.G., Synthesis of Carboxymethylated Nanocellulose Fabricated Ciprofloxacine – Montmorillonite Composite for Sustained Delivery of Antibiotics, International Journal of Pharmaceutics, 567: 118502 (2019).
[43] Dias E., Chalse H., Mutha S., Mundhe Y., Ambhore N., Kulkarni A., Mache A., Review on Synthetic/Natural Fibers Polymer Composite Filled with Nanoclay and Their Mechanical Performance, Materials Today: Proceedings, 77: 916-925 (2023).
[44] Ragunath S., Rathod M.L., Saravanan K.G., Rakesh N., Kifetew M., Optimization of Machining Parameters of Natural/Glass Fiber with Nanoclay Polymer Composite Using Response Surface Methodology, Journal of Nanomaterials, 2023: 9485769 (2023).
[45] Shahbaz A., Hussain N., Mahmood T., Iqbal H.M.N., Bin Emran T., Show P.L., Bilal M., 17 - Polymer Nanocomposites for Biomedical Applications, in: N. Ali, M. Bilal, A. Khan, T.A. Nguyen, R.K. Gupta (Eds.), Smart Polymer Nanocomposites, Elsevier, 379-394 (2023).
[46] Azzaoui K., Mejdoubi E., Lamhamdi A., Jodeh S., Hamed O., Berrabah M., Jerdioui S., Salghi R., Akartasse N., Errich A., Ríos Á., Zougagh M., Preparation and Characterization of Biodegradable Nanocomposites Derived from Carboxymethyl Cellulose and Hydroxyapatite, Carbohydrate Polymers, 167: 59-69 (2017).
[47] Pourmadadi M., Rahmani E., Shamsabadipour A., Samadi A., Esmaeili J., Arshad R., Rahdar A., Tavangarian F., Pandey S., Novel Carboxymethyl Cellulose Based Nanocomposite: A Promising Biomaterial for Biomedical Applications, Process Biochemistry, 130: 211-226 (2023).
[48] Mehrabi A., Jalise S.Z., Hivechi A., Habibi S., Kebria M.M., Haramshahi M.A., Latifi N., Karimi A., Milan P.B., Evaluation of Inherent Properties of the Carboxymethyl Cellulose (CMC) for Potential Application in Tissue Engineering Focusing on Bone Regeneration, Polymers for Advanced Technologies, 35(1): e6258 (2024).
[49] Okuda K., Shigemasa R., Hirota K., Mizutani T., In Situ Crystallization of Hydroxyapatite on Carboxymethyl Cellulose as a Biomimetic Approach to Biomass-Derived Composite Materials, ACS Omega, 7(14): 12127-12137 (2022).
[50] Hasan A., Waibhaw G., Saxena V., Pandey L.M., Nano-Biocomposite Scaffolds of Chitosan, Carboxymethyl Cellulose and Silver Nanoparticle Modified Cellulose Nanowhiskers for Bone Tissue Engineering Applications, International Journal of Biological Macromolecules, 111: 923-934 (2018).
[51] Palem R.R., Rao K.M., Shimoga G., Saratale R.G., Shinde S.K., Ghodake G.S., Lee S.-H., Physicochemical Characterization, Drug Release, and Biocompatibility Evaluation of Carboxymethyl Cellulose-Based Hydrogels Reinforced with Sepiolite Nanoclay, International Journal of Biological Macromolecules, 178: 464-476 (2021).
[52] Saberi Riseh R., Gholizadeh Vazvani M., Hassanisaadi M., Skorik Y.A., Micro-/Nano-Carboxymethyl Cellulose as a Promising Biopolymer with Prospects in the Agriculture Sector: A Review, Polymers, 15(2): 440 (2023).
[53] Sun X., Shen J., Yu D., Ouyang X.-k., Preparation of pH-Sensitive Fe3O4@C/Carboxymethyl Cellulose/Chitosan Composite Beads for Diclofenac Sodium Delivery, International Journal of Biological Macromolecules, 127: 594-605 (2019).
[54] Aminatun D., Hikmawati P., Widiyanti T., Amrillah A., Nia W., Firdania I.T., Abdullah C.A.C., Study of Mechanical and Thermal Properties in Nano-Hydroxyapatite/Chitosan/Carboxymethyl Cellulose Nanocomposite-Based Scaffold for Bone Tissue Engineering: The Roles of Carboxymethyl Cellulose, Applied Sciences, 10(19): 6970 (2020).
[55] Manjubala I., Basu P., Narendrakumar U., In Situ Synthesis of Hydroxyapatite/Carboxymethyl Cellulose Composites for Bone Regeneration Applications, Colloid and Polymer Science, 296(10): 1729-1737 (2018).
[56] Oprea M., Voicu S.I., Recent Advances in Applications of Cellulose Derivatives-Based Composite Membranes with Hydroxyapatite, Materials, 13(11): 2481 (2020).
[57] Altaani B.M., Almaaytah A.M., Dadou S., Alkhamis K., Daradka M.H., Hananeh W., Oral Delivery of Teriparatide Using a Nanoemulsion System: Design, in Vitro and in Vivo Evaluation, Pharmaceutical Research, 37(4): 80 (2020).
[58] Amani N., Javar H.A., Dorkoosh F.A., Rouini M.R., Amini M., Sharifzadeh M., Boumi S., Preparation and Pulsatile Release Evaluation of Teriparatide-Loaded Multilayer Implant Composed of Polyanhydride-Hydrogel Layers Using Spin Coating for the Treatment of Osteoporosis, Journal of Pharmaceutical Innovation, 16(2): 337-358 (2021).
[59] Liuyun J., Yubao L., Chengdong X., Preparation and Biological Properties of a Novel Composite Scaffold of Nano-Hydroxyapatite/Chitosan/Carboxymethyl Cellulose for Bone Tissue Engineering, Journal of Biomedical Science, 16(1): 65 (2009).
[60] Balakrishnan H., Husin M.R., Wahit M.U., Abdul Kadir M.R., Preparation and Characterization of Organically Modified Montmorillonite-Filled High Density Polyethylene/Hydroxyapatite Nanocomposites for Biomedical Applications, Polymer-Plastics Technology and Engineering, 53(8): 790-800 (2014).
[61] Garai S., Sinha A., Biomimetic Nanocomposites of Carboxymethyl Cellulose–Hydroxyapatite: Novel Three Dimensional Load Bearing Bone Grafts, Colloids and Surfaces B: Biointerfaces, 115: 182-190 (2014).
[62] Lamkhao S., Tandorn S., Thavornyutikarn P., Chokethawai K., Rujijanagul G., Thongkorn K., Jarupoom P., Randorn C., Synergistic Amalgamation of Shellac with Self-Antibacterial Hydroxyapatite and Carboxymethyl Cellulose: An Interactive Wound Dressing for Ensuring Safety and Efficacy in Preliminary in Vivo Studies, International Journal of Biological Macromolecules, 253: 126809 (2023).
[63] Kar S., Kaur T., Thirugnanam A., Microwave-Assisted Synthesis of Porous Chitosan–Modified Montmorillonite–Hydroxyapatite Composite Scaffolds, International Journal of Biological Macromolecules, 82: 628-636 (2016).
[64] Mahdavinia G.R., Afzali A., Etemadi H., Hoseinzadeh H., Magnetic/pH-Sensitive Nanocomposite Hydrogel Based Carboxymethyl Cellulose–G-Polyacrylamide/Montmorillonite for Colon Targeted Drug Delivery, Nanomedicine Research Journal, 2(2): 111-122 (2017).
[65] Ahmadi M., Pourmadadi M., Ghorbanian S.A., Yazdian F., Rashedi H., Ultra pH-Sensitive Nanocarrier Based on Fe2O3/Chitosan/Montmorillonite for Quercetin Delivery, International Journal of Biological Macromolecules, 191: 738-745 (2021).
[66] Heragh B.K., Javanshir S., Mahdavinia G.R., Jamal M.R.N., Hydroxyapatite Grafted Chitosan/Laponite RD Hydrogel: Evaluation of the Encapsulation Capacity, pH-Responsivity, and Controlled Release Behavior, International Journal of Biological Macromolecules, 190: 351-359 (2021).
[67] Saadatjoo N., Golshekan M., Shariati S., Azizi P., Nemati F., Ultrasound-Assisted Synthesis of β-Amino Ketones Via a Mannich Reaction Catalyzed by Fe3O4 Magnetite Nanoparticles as an Efficient, Recyclable and Heterogeneous Catalyst, Arabian Journal of Chemistry, 10: S735-S741 (2017).
[68] Saadatjoo N., Golshekan M., Shariati S., Kefayati H., Azizi P., Organic/Inorganic MCM-41 Magnetite Nanocomposite as a Solid Acid Catalyst for Synthesis of Benzo[α]Xanthenone Derivatives, Journal of Molecular Catalysis A: Chemical, 377: 173-179 (2013).
[69] Mahdi Eshaghi M., Pourmadadi M., Rahdar A., Díez-Pascual A.M., Novel Carboxymethyl Cellulose-Based Hydrogel with Core–Shell Fe3O4@SiO2 Nanoparticles for Quercetin Delivery, Materials, 15(24): 8711 (2022).
[70] Xie W., Zang X., Immobilized Lipase on Core–Shell Structured Fe3O4–MCM-41 Nanocomposites as a Magnetically Recyclable Biocatalyst for Interesterification of Soybean oil and Lard, Food Chemistry, 194: 1283-1292 (2016).
[71] Hosseini H.R., Abdouss M., Golshekan M., Hydroxyapatite Incorporated with Fe3O4@MCM-41 Core–Shell: A Promising Nanocomposite for Teriparatide Delivery in Bone Tissue Regeneration, ACS Omega, (2023).
[72] Abbas M., Parvatheeswara Rao B., Nazrul Islam M., Naga S.M., Takahashi M., Kim C., Highly Stable- Silica Encapsulating Magnetite Nanoparticles (Fe3O4/SiO2) Synthesized Using Single Surfactantless- Polyol Process, Ceramics International, 40(1, Part B): 1379-1385 (2014).
[73] Zakharov N.A., Ezhova Z.A., Koval’ E.M., Kalinnikov V.T., Chalykh A.E., Hydroxyapatite-Carboxymethyl Cellulose Nanocomposite Biomaterial, Inorganic Materials, 41(5): 509-515 (2005).
[74] Adinugraha M.P., Marseno D.W., Haryadi, Synthesis and Characterization of Sodium Carboxymethylcellulose from Cavendish Banana Pseudo Stem (Musa Cavendishii LAMBERT), Carbohydrate Polymers, 62(2): 164-169 (2005).
[75] Nazeer M.A., Yilgör E., Yilgör I., Intercalated Chitosan/Hydroxyapatite Nanocomposites: Promising Materials for Bone Tissue Engineering Applications, Carbohydrate Polymers, 175: 38-46 (2017).
[76] Guo X., Yan H., Zhao S., Li Z., Li Y., Liang X., Effect of Calcining Temperature on Particle Size of Hydroxyapatite Synthesized by Solid-State Reaction at Room Temperature, Advanced Powder Technology, 24(6): 1034-1038 (2013).
[77] Narayanan D., Anitha A., Jayakumar R., Nair S.V., Chennazhi K.P., Synthesis, Characterization and Preliminary In Vitro Evaluation of PTH 1-34 Loaded Chitosan Nanoparticles for Osteoporosis, Journal of Biomedical Nanotechnology, 8(1): 98-106 (2012).
[78] Tariq S., Raza A.R., Khalid M., Rubab S.L., Khan M.U., Ali A., Tahir M.N., Braga A.A.C., Synthesis and Structural Analysis of Novel Indole Derivatives by XRD, Spectroscopic and DFT Studies, Journal of Molecular Structure, 1203: 127438 (2020).
[79] Laabd M., Brahmi Y., El Ibrahimi B., Hsini A., Toufik E., Abdellaoui Y., Abou Oualid H., El Ouardi M., Albourine A., A Novel Mesoporous Hydroxyapatite@Montmorillonite Hybrid Composite for High-Performance Removal of Emerging Ciprofloxacin Antibiotic from Water: Integrated Experimental and Monte Carlo Computational Assessment, Journal of Molecular Liquids, 338: 116705 (2021).
[80] Yadollahi M., Gholamali I., Namazi H., Aghazadeh M., Synthesis and Characterization of Antibacterial Carboxymethylcellulose/CuO Bio-Nanocomposite Hydrogels, International Journal of Biological Macromolecules, 73: 109-114 (2015).
[81] Rajabzadeh-Khosroshahi M., Pourmadadi M., Yazdian F., Rashedi H., Navaei-Nigjeh M., Rasekh B., Chitosan/Agarose/Graphitic Carbon Nitride Nanocomposite as an Efficient pH-Sensitive Drug Delivery System for Anticancer Curcumin Releasing, Journal of Drug Delivery Science and Technology, 74: 103443 (2022).
[82] Joseph E., Singhvi G., Chapter 4 - Multifunctional Nanocrystals for Cancer Therapy: A Potential Nanocarrier, in: A.M. Grumezescu (Ed.), »Nanomaterials for Drug Delivery and Therapy«, William Andrew Publishing, 91-116 (2019).
[83] Mohebali A., Abdouss M., Afshar Taromi F., Fabrication Of Biocompatible Antibacterial Nanowafers Based On HNT/PVA Nanocomposites Loaded With Minocycline For Burn Wound Dressing, Materials Science and Engineering, C 110: 110685 (2020).
[84] Abdollahi E., Khalafi-Nezhad A., Mohammadi A., Abdouss M., Salami-Kalajahi M., Synthesis of New Molecularly Imprinted Polymer Via Reversible Addition Fragmentation Transfer Polymerization as a Drug Delivery System, Polymer, 143: 245-257 (2018).
[85] Strach A., Dulski M., Wasilkowski D., Matus K., Dudek K., Podwórny J., Rawicka P., Grebnevs V., Waloszczyk N., Nowak A., Poloczek P., Golba S., Multifaceted Assessment of Porous Silica Nanocomposites: Unraveling Physical, Structural, and Biological Transformations Induced by Microwave Field Modification, Nanomaterials, 14(4): 337 (2024).
[86] Chen X., Chen J., Huang N., The Structure, Formation, and Effect of Plasma Protein Layer on the Blood Contact Materials: A Review, Biosurface and Biotribology 8(1): 1-14 (2022).
[87] Eswaramoorthy R., Chang C.-C., Wu S.-C., Wang G.-J., Chang J.-K., Ho M.-L., Sustained release of PTH(1–34) from PLGA microspheres suppresses osteoarthritis progression in Rats, Acta Biomaterialia, 8(6): 2254-2262 (2012).
[88] Keegan M.T., 36 - Endocrine Pharmacology, in: H.C. Hemmings, T.D. Egan (Eds.), »Pharmacology and Physiology for Anesthesia (Second Edition)«, Elsevier, Philadelphia, 708-731 (2019).
[89] Samadi A., Pourmadadi M., Yazdian F., Rashedi H., Navaei-Nigjeh M., Eufrasio-da-silva T., Ameliorating Quercetin Constraints in Cancer Therapy with pH-Responsive Agarose-Polyvinylpyrrolidone -Hydroxyapatite Nanocomposite Encapsulated in Double Nanoemulsion, International Journal of Biological Macromolecules, 182: 11-25 (2021).
[90] Liu Z.-S., Wen J., Huang C.-Y., Zhang P.-W., Miao Y.-L., Cheng H., Li S.-Y., Nanomedicines Based on Responsive Nanocarriers for Cancer Therapy, Advanced Therapeutics, 7(2): 2300223 (2024).