Nashrieh Shimi va Mohandesi Shimi Iran

Nashrieh Shimi va Mohandesi Shimi Iran

biocompatibility investigation of hydroxyapatite/chitosan nanocomposites doped by metal oxides

Document Type : Research Article

Authors
Alireza Esmaeilzadeh
saeede haghbin-lasht neshani
felora Heshmatpour
Department of Inorganic Chemistry, Faculty of Chemistry, Khajeh Nasir al-Din Tusi University of Technology, Tehran, Iran
Abstract
Nano Hydroxyapatite (nHA) composites have been widely used in bone regeneration due to their unique properties. In this paper, doped nHA composites with doping of MgO, ZnO, and CeO2 metal oxides into nHA structure were successfully attained using precipitation method. Also, chitosan (CS) was added to the nHA composites to improve the biocompatibility. As the results showed, doping the metal oxides into the nHA structure led to the change of the lattice parameters in nHA structure. Finally, bioactivity of the composites was investigated by MTT assay by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide. MTT assay is shown that doped-nHA structure gives acceptable cytotoxicity against the MG-63 cell. 
Keywords
Hydroxyapatite
Metal oxide
Bioactivity
Cytotoxicity

Subjects

[1] Mohamed K.R., Beherei H.H., El Bassyouni G.T., El Mahallawy N., Fabrication and Mechanical Evaluation of Hydroxyapatite/Oxide Nano-Composite Materials, Materials Science & Engineering C, 33: 4126–4132 (2013)
[2] Abidi S.S.A., Murtaza Q., Synthesis and Characterization of Nano-Hydroxyapatite Powder using Wet Chemical Precipitation Reaction, Journal of Materials Science & Technology, 30: 307–310 (2014)
[3] Bhuiyan D., Jablonsky M. J., Kolesov I., Middleton J., Wick T. M., Tannenbaum R, Novel Synthesis and Characterization of a Collagen-based Biopolymer Initiated by Hydroxyapatite Nanoparticles, Acta Biomaterialia, 15: 181–190 (2015)
[4] Balakrishnan B., Soman D., Payanam U., Laurent A., Labarre D., Jayakrishnan A, A Novel Injectable Tissue Adhesive based on Oxidized Dextran and Chitosan, Acta Biomaterialia, 53: 343–354 (2017)
[5] Šupová M., Substituted Hydroxyapatites for Biomedical Applications: A Review, Ceramics International, 41: 9203–9231(2015)
[6] Bertinetti L., Tampieri A., Landi E., Martra G., Coluccia S, Punctual Investigation of Surface Sites of HA and Magnesium-HA, Journal of the European Ceramic Society, 26(6): 987–991 (2006)
[7] Laurencin D., Almora-Barrios N., de Leeuw N. H., Gervais C., Bonhomme C., Mauri F., Chrzanowski W., Knowles J. C., Newport R. J., Wong A., Gan Z., Smith M. E., Magnesium Incorporation into Hydroxyapatite, Biomaterials, 32: 1826–1837(2011)
[8] Dizaj S. M., Lotfipour F., Barzegar-Jalali M., Zarrintan M. H., Adibkia K., Antimicrobial Activity of the Metals and Metal Oxide Nanoparticles, Materials Science & Engineering C, 44: 278–84 (2014)
[9] Gnaneshwar P. V., Sudakaran S. V., Abisegapriyan S., Sherine J., Ramakrishna S., Ab. Rahim M. H., Mohd. Yusoff M., Jose R., Venugopal J. R., Ramification of Zinc Oxide Doped Hydroxyapatite Biocomposites for the Mineralization of Osteoblasts, Materials Science & Engineering C, 96: 337–346 (2019)
[10] Vormann J., Magnesium: Nutrition and Metabolism, Molecular Aspects of Medicine, 24:27–37(2003)
[11] Jin T., He Y., Antibacterial Activities of Magnesium Oxide (MgO) Nanoparticles Against Foodborne Pathogens. Journal of Nanoparticle Research, 13(12): 6877–6885 (2011)
[12] Coelho C. C., Araújo R., Quadros P. A., Sousa S. R., Monteiro F. J., Antibacterial Bone Substitute of Hydroxyapatite and Magnesium Oxide to Prevent Dental and Orthopaedic Infections, Materials Science & Engineering C, 97: 529–538 (2019)
[13] Gopi D., Ramya S., Rajeswari D., Karthikeyan P., Kavitha L., Strontium, Cerium Co-Substituted Hydroxyapatite Nanoparticles: Synthesis, Characterization, Antibac-Terial Activity Towards Prokaryotic Strains and in Vitro Studies. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 451: 172-180 (2014)
[14] Li J., Zhu D., Yin J., Liu Y., Yao F., Yao K., Formation of Nano-Hydroxyapatite Crystal in Situ in Chitosanpectin Polyelectrolyte Complex Network. Materials Science & Engineering C, 30: 795–803 (2010)
[15] Ibrahim D., Mostafa A.A., Korowash S.l., Chemical Characterization of some Substituted Hydroxyapatites, Chemistry Central Journal, 5: 74 (2011)
[16] Frasnelli M., Cristofaro F., Sglavo V.M., Dirè S., Callone E., Ceccato R., Bruni G., Cornaglia A.I., Visai L., Synthesis and Characterization of Strontium-Substituted Hydroxyapatite Nanoparticles for Bone Regeneration, Materials Science & Engineering C, 71: 653–662 (2017)
[17] Nguyen, N.K., Leoni, M., Maniglio, D. and Migliaresi, C., Hydroxyapatite Nanorods: Soft-Template Synthesis, Characterization and Preliminary in Vitro Tests. Journal of biomaterials applications, 28: 49-61 (2013)
[18] Virginia, M., Laksono, A.D., Asih, W.P.K. and Agustiningtyas, D.T., Study on Biocompatibility of Chitosan/Hydroxyapatite Doped Silicon Composite as Material for Alveolar Socket Preservation. In Journal of Physics: Conference Series, 1726(1): 012007 (2021)
[19] Sun, F., Koh, K., Ryu, S.C., Han, D.W. and Lee, J., Biocompatibility of Nanoscale Hydroxyapatite-Embedded Chitosan Films. Bulletin of the Korean Chemical Society, 33: 3950-3956. (2012)
[20] Li, M., Wang, Y., Liu, Q., Li, Q., Cheng, Y., Zheng, Y., Xi, T. and Wei, S., In Situ Synthesis and Biocompatibility of Nano Hydroxyapatite on Pristine and Chitosan Functionalized Graphene Oxide. Journal of Materials Chemistry B, 1(4): 475-484 (2013)
[21] Kazimierczak, P., Benko, A., Nocun, M. and Przekora, A., Novel Chitosan/Agarose/Hydroxyapatite Nanocomposite Scaffold for Bone Tissue Engineering Applications: Comprehensive Evaluation of Biocompatibility and Osteoinductivity with the use of Osteoblasts and Mesenchymal Stem Cells. International journal of nanomedicine, 14: 6615. (2019)