Nashrieh Shimi va Mohandesi Shimi Iran

Nashrieh Shimi va Mohandesi Shimi Iran

Experimental and computational studies of thiolation of oligonucleotide towards its stabilizing on the surface of silver nanoparticles

Document Type : Research Article

Authors
Elaheh Aali
Ali Shokuhi Rad
Mehry Esfahanian
Qaemshahr Branch, Islamic Azad University, Qaemshahr, I.R. IRAN
Abstract
In this study, a specific sequence of oligonucleotides was attached to silver nanoparticles after thiolization in a saline medium. Investigation of this bond was performed using ultraviolet-visible spectroscopy (UV_Vis spectroscopy) by changing the silver nanoparticles' wavelength and thiol oligonucleotide attached to silver nanoparticles. Also, by bond transfer in polyacrylamide gel electrophoresis (PAGE) due to the increase in molecular weight after binding silver nanoparticles to a thiolated oligonucleotide, this bond's accuracy has been investigated. To find the bonds' electronic structure and stability, DFT studies have been performed using the Gaussian 09 program, aiming to see the optimal conditions for the binding of thiolated oligonucleotides to silver nanoparticles. In general, the binding of silver nanoparticles to thiolated oligonucleotides makes the specific adsorption of the sequence to the cell more targeted. Also, it increases the accuracy, precision, and specificity of adsorption. Calculations showed the absorption energy of -54.4 kJ / mol, the transfer of electric charge of 0.129 e after binding the thiolated oligonucleotide to silver nanoparticles, indicating a useful connection between them.
Keywords
Oligonucleotide
Purification
Silver nanoparticles
Thiol
Quantum mechanics

Subjects

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