Nashrieh Shimi va Mohandesi Shimi Iran

Nashrieh Shimi va Mohandesi Shimi Iran

A Sensitive, Simple, and Direct Determination of β-Blocker Carvedilol Drug at Trace Level in Biological Samples by Using Tb(III)-1,10-Phenanthroline-Silver Nanoparticles Luminescent Nanosensor Probe

Document Type : Research Article

Authors
Masoomeh Shaghaghi 1
Samaneh Rashtbari 2
Gholam Reza Dehghan 2
Seyedeh Hanieh Seyed Mahdavi Agdam 1
1 Department of Chemistry, Payame Noor University, Tehran, I.R. IRAN
2 Department of Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, I.R. IRAN
Abstract
A new, sensitive, simple, and rapid fluorometric method was developed for the direct determination of carvedilol in real samples. The method was based on the inhibition of the fluorescence resonance energy transfer (FRET) process between terbium (III)-1, 10-phenanthroline (Tb-phen) complex, and silver nanoparticles (AgNPs). For this purpose, the quenching effect of AgNPs in the concentration range of 0.9×10-12 – 5.4 ×10-11 M was investigated on the Tb-phen complex fluorescence intensity in the excitation and emission wavelengths of 300 and 500 nm, respectively. The results showed that the amount of AgNPs quenching, as a result of FRET process, is very high and there is a good linear relationship between the quenched fluorescence intensity and AgNPs concentration. The quenching probably occurs when the Tb-phen complex is attached to the AgNPs by electrostatic force and long-range dipole-dipole interaction between the excited donor (Tb-phen complex) and ground-state acceptor molecules (AgNPs). Upon the addition of carvedilol, the quenched fluorescence of Tb-phen complex was gradually recovered by carvedilol via its strong adsorption on the surface of AgNPs and removal of Tb-phen complex from the AgNPs surface (turn off/on process). Under the optimum conditions, a linear relationship was obtained between the enhanced fluorescence intensity and the carvedilol concentration in the range of (5-1000)×10-8 M with the detection limit of 2.0×10-8 M. The proposed method was successfully applied to determine carvedilol in the spiked normal serum samples and analytical recoveries from treated serum samples were in the range of 92.5–103% and relative standard deviation (RSD%) were 0.14-1.15.
Keywords
Tb-phen complex
Determination of carvedilol
FRET-based nanosensor
Turn off-on

Subjects

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