Nashrieh Shimi va Mohandesi Shimi Iran

Nashrieh Shimi va Mohandesi Shimi Iran

A Review of Thermogel with an Applied Approach in Medical Engineering

Document Type : Review Article

Authors
Department of Medical Engineering, Faculty of Engineering, Maybod University, Yazd, Iran
Abstract
Thermogels, having unique sol-gel phase transition properties, have recently received much attention. These types of hydrogels show a phase transition when exposed to temperature changes. This feature is the key to the application of thermogels in medicine. The thermogelling behavior is the result of a combination of multiple heat response mechanisms. These mechanisms include the behavior of the lowest critical temperature of the solution, micellization and micellar aggregation of thermogel-forming polymers. Thermogels have wide applications in medical engineering and especially in tissue engineering. Injectability is one of the most important properties of thermogels for use in tissue engineering, which induces high compatibility, easy loading of cells and growth factors, and help in tissue regeneration to these hydrogels. In recent years, the use of thermogels in drug delivery and the study of thermogel drug delivery systems have become very common. Thermogels can deliver the drug to the desired location in a targeted manner and increase the life of the drug by protecting the drug in the body. Thermogels have also been considered as a suitable cell culture medium in various researches due to their three-dimensional porous structure. In addition, these materials have been used as self-cleaning membranes due to their ability to reduce fibrous encapsulation and facilitate analyte diffusion in biosensors. In this article, an attempt was made to review the research conducted in the field of functional mechanisms of thermogels and the progress achieved in this field. In the following, with a practical attitude, the research conducted in the field of using thermogels in medical engineering, such as use in drug delivery, tissue engineering, 3D culture of cells/stem cells, and membranes and sensors, was examined.
Keywords

Subjects


[1]  Amiri, F., Sabzevari, A., Kabiri, K., Bouhendi, H., Siahkamari, M., Conversion Lignocellulosic Bagasse Biomass into HydrogelIranian Journal of Polymer Science and Technology, 29(5): 453-465 (2017).
[2] Sabzevari, A., Kabiri, K., Converting Date Seed Biomass Into Highly Absorbing HydrogelIranian Polymer Journal, 25: 597-606 (2016).
[۳] رضانژاد بردجی، قاسم، حسینی سمانه سادات، سنتز هیدروژل نانوکامپوزیت آهن و بررسی رهایش داروی ضدسرطان دوکسوروبیسین، نشریه شیمی و مهندسی شیمی ایران، (۱)۳۸: ۸۹-۱۰۸ (۱۳۹۸).
[4] Sabzevari, A., Rayat Pisheh, H., Ansari, M., Salati, A., Progress in Bioprinting Technology for Tissue RegenerationJournal of Artificial Organs,1-20 (2023).
[5] Ansari, M., Meftahizadeh, H., Eslami, H., Physical and Antibacterial Properties of Chitosan-Guar-Peppermint gel for Improving Wound HealingPolymer Bulletin, 80(7): 8133-8149 (2023).
[6] Ansari, M., Meftahizadeh, H., Eslami, H., Fabrication of Multifunctional Chitosan-Guar-Aloe Vera gel to Promote Wound HealingChemical Papers, 76(3): 1513-1524 (2022).
[8] Xue, K., Liow, S.S., Karim, A.A., Li, Z., Loh, X.J., A recent Perspective on Noncovalently Formed Polymeric HydrogelsThe Chemical Record, 18(10): 1517-1529 (2018).
[9] Webber, M.J., Appel, E.A., Meijer, E.W., Langer, R., Supramolecular Biomaterials. Nature materials, 15(1): 13-26 (2016).
[10] Lim, J.Y.C., Lin, Q., Xue, K., Loh, X.J., Recent Advances in Supramolecular Hydrogels for Biomedical ApplicationsMaterials Today Advances, 3: 100021 (2019).
[11] Koons, G.L., Diba, M., Mikos, A.G., Materials Design for Bone-Tissue EngineeringNature Reviews Materials, 5(8): 584-603 (2020).
[12] Montoya, C., Du, Y., Gianforcaro, A.L., Orrego, S., Yang, M., Lelkes, P.I., On the Road to Smart Biomaterials for Bone Research: Definitions, Concepts, Advances, and OutlookBone Research, 9(1): 12 (2021).
[13] Dimatteo, R., Darling, N.J., Segura, T., In Situ Forming Injectable Hydrogels for Drug Delivery and Wound Repair. Advanced drug delivery reviews, 127: 167-184 (2018).
[14] Kim, Y.J., Matsunaga, Y.T., Thermo-Responsive Polymers And Their Application As Smart BiomaterialsJournal of Materials Chemistry B, 5(23): 4307-4321 (2017).
[15] Liow, S.S., Dou, Q., Kai, D., Karim, A.A., Zhang, K., Xu, F., Loh, X.J., Thermogels: In Situ Gelling BiomaterialACS Biomaterials Science & Engineering, 2(3): 295-316 (2016).
[16] Nele, V., Wojciechowski, J.P., Armstrong, J.P., Stevens, M.M.,Tailoring Gelation Mechanisms for Advanced Hydrogel ApplicationsAdvanced Functional Materials, 30(42): 2002759 (2020).
[17] Akash, M.S.H., Rehman, K., Recent Progress in Biomedical Applications of Pluronic (PF127): PHARMACEUTICAL PErspectivesJournal of Controlled Release, 209: 120-138 (2017).
[19] Darge, H.F., Andrgie, A.T., Tsai, H.C., Lai, J.Y., Polysaccharide and Polypeptide Based Injectable Thermo-Sensitive Hydrogels for Local Biomedical ApplicationsInternational journal of biological macromolecules, 133: 545-563 (2019).
[20] Aswathy, S.H., Narendrakumar, U., Manjubala, I., Commercial Hydrogels for Biomedical applicationsHeliyon, 6(4): (2019).
[21] Patel, M., Lee, H.J., Park, S., Kim, Y., Jeong, B., Injectable Thermogel for 3D Culture of Stem Cells. Biomaterials, 159: 91-107 (2018).
[22] Chee, P.L., Young, D.J., Loh, X.J., Degradation Behaviour of Biodegradable ThermogelsBiodegradable Thermogels, 2: 113 (2018).
[23] Chen, J., Xie, F., Li, X., Chen, L., Ionic Liquids for the Preparation of Biopolymer Materials for Drug/Gene Delivery: A ReviewGreen Chemistry, 20(18): 4169-4200 (2018).
[24] Sinawang, G., Osaki, M., Takashima, Y., Yamaguchi, H., Harada, A., Supramolecular Self-Healing Materials from Non-Covalent Cross-Linking Host–Guest Interactions. Chemical Communications, 56(32): 4381-4395 (2020).
[25] Bordat, A., Boissenot, T., Nicolas, J., Tsapis, N.,Thermoresponsive Polymer Nanocarriers for Biomedical ApplicationsAdvanced drug delivery reviews, 138: 167-192 (2019).
[26] Zhang, Q., Weber, C., Schubert, U.S., Hoogenboom, R., Thermoresponsive Polymers with Lower Critical Solution Temperature: From Fundamental Aspects and Measuring Techniques to Recommended Turbidimetry ConditionsMaterials Horizons, 4(2): 109-116 (2017).
[27] Pasparakis, G., Tsitsilianis, C., LCST Polymers: Thermoresponsive Nanostructured Assemblies Towards BioapplicationsPolymer, 211: 123146 (2020).
[28] Shibayama, M., Li, X., Sakai, T., Precision Polymer Network Science with Tetra-PEG Gels—a Decade History and FutureColloid and Polymer Science, 297: 1-12 (2019).
[29] Brewer, K., Gundsambuu, B., Facal Marina, P., Barry, S.C., Blencowe, A., Thermoresponsive Poly (ε-Caprolactone)-Poly (Ethylene/Propylene Glycol) Copolymers as Injectable Hydrogels for Cell TherapiesPolymers, 12(2): 367 (2020).
[30] Hua, M., Wu, D., Wu, S., Ma, Y., Alsaid, Y., He, X.,4D Printable Tough and Thermoresponsive HydrogelsACS applied materials & interfaces, 13(11): 12689-12697 (2020).
[31] Ashraf, S., Park, H.K., Park, H., Lee, S.H., Snapshot of Phase Transition in Thermoresponsive Hydrogel PNIPAM: Role in Drug Delivery and Tissue EngineeringMacromolecular Research, 24: 297-304 (2016).
[32] Gandhi, A., Paul, A., Sen, S.O., Sen, K.K., Studies on Thermoresponsive Polymers: Phase Behaviour, Drug Delivery and Biomedical Applicationsasian journal of pharmaceutical sciences, 10(2): 99-107 (2015).
[33] Torres-Figueroa, A.V., Pérez-Martínez, C.J., Encinas, J.C., Burruel-Ibarra, S., Silvas-García, M.I., García Alegría, A.M., del Castillo-Castro, T., Thermosensitive Bioadhesive Hydrogels Based on Poly (N-Isopropylacrilamide) and Poly (Methyl Vinyl Ether-Alt-Maleic Anhydride) for the Controlled Release of Metronidazole in the Vaginal EnvironmentPharmaceutics, 13(8): 1284 (2021).
[34] Mohammed, M.N., Yusoh, K.B., Shariffuddin, J.H.B.H., Poly (N-Vinyl Caprolactam) Thermoresponsive Polymer in Novel Drug Delivery Systems: A ReviewMaterials Express, 8(1): 21-34 (2018).
[35] Rao, K.M., Rao, K.S.V.K., Ha, C.S., Stimuli Responsive Poly (Vinyl Caprolactam) Gels for biomedical applicationsGels, 2(1): 6 (2016).
[36] Halperin, A., Kröger, M., Winnik, F.M., Poly (N‐Isopropylacrylamide) Phase Diagrams: Fifty Years of ResearchAngewandte Chemie International Edition, 54(51): 15342-15367 (2015).
[37] Inoue, M., Hayashi, T., Hikiri, S., Ikeguchi, M., Kinoshita, M., Mechanism Of Globule-To-Coil Transition Of Poly (N-Isopropylacrylamide) In Water: Relevance To Cold Denaturation Of A ProteinJournal of Molecular Liquids, 292: 111374 (2019).
[38] de Oliveira, T.E., Mukherji, D., Kremer, K., Netz, P.A.,Effects of Stereochemistry and Copolymerization on the LCST of PNIPAmThe Journal of chemical physics, 146(3): (2017).
[39] Polák, J., Ondo, D., Heyda, J., Thermodynamics of  N-Isopropylacrylamide in Water: Insight from Experiments, Simulations, and Kirkwood–Buff Analysis TeamworkThe Journal of Physical Chemistry B, 124(12): 2495-2504 (2020).
[40] Hu, Y., Barbier, L., Li, Z., Ji, X., Le Blay, H., Liu, J., Lam, J.W., Marcellan, A., Tang, B.Z., Making Hydrogels Stronger through Hydrophilicity-Hydrophobicity Transformation, Thermoresponsive Morphomechanics and Crack Multifurcation, (2020).
[41] Burek, M., Waśkiewicz, S., Awietjan, S., Wandzik, I., Thermoresponsive Hydrogels with Covalently Incorporated Trehalose as Protein CarriersReactive and Functional Polymers, 119: 105-115 (2017).
[42] Smith, A.A., Maikawa, C.L., Hernandez, H.L., Appel, E.A., Controlling properties of Thermogels by Tuning Critical Solution Behaviour of Ternary CopolymersPolymer Chemistry, 12(13): 1918-1923 (2021)
[43] Bayat, N., Zhang, Y., Falabella, P., Menefee, R., Whalen III, J.J., Humayun, M.S., Thompson, M.E., A Reversible Thermoresponsive Sealant for Temporary Closure of Ocular TraumaScience translational medicine, 9(419): eaan3879 (2017).
[44] Tang, L., Wang, L., Yang, X., Feng, Y., Li, Y., Feng, W., Poly (N-Isopropylacrylamide)-Based Smart Hydrogels: Design, Properties and ApplicationsProgress in Materials Science, 115: 100702 (2021).
[45] Koetting, M.C., Peters, J.T., Steichen, S.D., Peppas, N.A., Stimulus-Responsive Hydrogels: Theory, Modern Advances, and ApplicationsMaterials Science and Engineering: R: Reports, 93: 1-49 (2015).
[46] Chou, P.Y., Chen, S.H., Chen, C.H., Chen, S.H., Fong, Y.T., Chen, J.P., Thermo-Responsive in-situ Forming Hydrogels as Barriers to Prevent Post-Operative Peritendinous AdhesionActa Biomaterialia, 63: 85-95 (2017).
[47] Luo, Z., Xue, K., Zhang, X., Lim, J.Y., Lai, X., Young, D.J., Zhang, Z.X., Wu, Y.L., Loh, X.J., Thermogelling Chitosan-Based Polymers for the Treatment of Oral Mucosa UlcersBiomaterials science, 8(5): 1364-1379 (2020).
[48] Cao, M., Wang, Y., Hu, X., Gong, H., Li, R., Cox, H., Zhang, J., Waigh, T.A., Xu, H., Lu, J.R., Reversible Thermoresponsive Peptide–PNIPAM Hydrogels for Controlled Drug DeliveryBiomacromolecules, 20(9): 3601-3610 (2019).
[50] Bates, C.M., Bates, F.S., 50th Anniversary Perspective: Block Polymers Pure PotentialMacromolecules, 50(1): 3-22 (2017).
[51] Park, J., Jang, S., Kon Kim, J., Morphology and Microphase Separation of star CopolymersJournal of Polymer Science Part B: Polymer Physics, 53(1): 1-21 (2015).
[52] Zhang, Z., Wang, Z., Wang, Q., Zhang, H., Tang, Y., Qu, Q., Shen, L., Xiang, D., Mi, Y., Yan, X., Synthesis of a Multi-Branched Trinuclear Ionic Liquid Demulsifier and Evaluation of its Performance in W/O EmulsionsFuel, 338: 127188 (2022).
[54] Zhang, K., Xue, K., Loh, X.J., Thermo-Responsive Hydrogels: From Recent Progress to Biomedical ApplicationsGels, 7(3): 77 (2021).
[55] Cook, M.T., Haddow, P., Kirton, S.B., McAuley, W.J., Polymers Exhibiting Lower Critical Solution Temperatures as a Route to Thermoreversible Gelators for HealthcareAdvanced Functional Materials, 31(8): 2008123 (2021).
[56] Lin, Q., Lim, J.Y., Xue, K., Chee, C.P., Loh, X.J., Supramolecular Thermogels from Branched PCL-containing polyurethanesRSC advances, 10(64): 39109-39120 (2020).
[57] Arranja, A., Waton, G., Schosseler, F., Mendes, E., Lack of a Unique Kinetic Pathway in the Growth and Decay of Pluronic MicellesSoft Matter, 12(3): 769-778 (2016).
[58] Figueroa-Ochoa, E.B., Bravo-Anaya, L.M., Vaca-López, R., Landázuri-Gómez, G., Rosales-Rivera, L.C., Diaz-Vidal, T., Carvajal, F., Macías-Balleza, E.R., Rharbi, Y., Soltero-Martínez, J.F.A., Structural Behavior of Amphiphilic Triblock Copolymer P104/Water SystemPolymers, 15(11): 2551 (2023).
[59] Puig-Rigall, J., Obregon-Gomez, I., Monreal-Pérez, P., Radulescu, A., Blanco-Prieto, M.J., Dreiss, C.A., González-Gaitano, G., Phase Behaviour, Micellar Structure and Linear Rheology of Tetrablock Copolymer Tetronic 908Journal of colloid and interface science, 524: 42-51 (2018).
[60] Lu, Y., Yue, Z., Xie, J., Wang, W., Zhu, H., Zhang, E., Cao, Z., Micelles with Ultralow Critical Micelle Concentration as Carriers for Drug DeliveryNature biomedical engineering, 2(5): 318-325 (2018).
[61] Zinn, T., Willner, L., Pipich, V., Richter, D., Lund, R., Molecular Exchange Kinetics of Micelles: Corona Chain Length DependenceACS Macro Letters, 5(7): 884-888 (2016).
[62] Loh, X.J., Biodegradable Thermogelling Polymers for Drug DeliveryBiodegradable Thermogels, 2: 76 (2018).
[63] Ghezzi, M., Pescina, S., Padula, C., Santi, P., Del Favero, E., Cantù, L., Nicoli, S., Polymeric Micelles in Drug Delivery: An Insight of the Techniques for Their Characterization and Assessment in Biorelevant ConditionsJournal of Controlled Release, 332: 312-336 (2021).
[64] Lu, Y., Lin, J., Wang, L., Zhang, L., Cai, C., Self-Assembly of Copolymer Micelles: Higher-Level Assembly for Constructing Hierarchical StructureChemical reviews, 120(9): 4111-4140 (2020).
[66] Huang, P., Song, H., Zhang, Y., Liu, J., Cheng, Z., Liang, X.J., Wang, W., Kong, D., Liu, J., FRET-Enabled Monitoring of the Thermosensitive Nanoscale Assembly of Polymeric Micelles Into Macroscale Hydrogel and Sequential Cognate Micelles ReleaseBiomaterials, 145: 81-91 (2017).
[67] Cui, S., Yu, L., Ding, J., Semi-Bald Micelles and Corresponding Percolated Micelle Networks of ThermogelsMacromolecules, 51(16): 6405-6420 (2018).
[68] Cui, S., Yu, L., Ding, J., Thermogelling of Amphiphilic Block Copolymers in Water: ABA Type Versus AB or BAB TypeMacromolecules, 52(10): 3697-3715 (2019).
[۶۹] مسلم توکل، محمد امین محمدی­فر، مروری بر صمغ کتیرا و استفاده از آن در زیست پزشکی، نشریه شیمی و مهندسی شیمی ایران، (۲)36: 20-1 (۱۳۹۶).
[70] Wei, Z., Volkova, E., Blatchley, M.R., Gerecht, S., Hydrogel Vehicles for Sequential Delivery of Protein Drugs to Promote Vascular RegenerationAdvanced drug delivery reviews, 149: 95-106 (2019).
[71] Yang, R., Sabharwal, V., Shlykova, N., Okonkwo, O.S., Pelton, S.I., Kohane, D.S., Treatment of STREPTOCOCCUS PNEUMONIAE OTITIS MEDIA in a Chinchilla Model by Transtympanic Delivery of AntibioticsJCI insight, 3(19): (2018).
[72] Rezanejade Bardajee, G., Asgari, S., Mirshokraie, S.A., Submicron Particles of Double Network Alginate/Polyacrylamide Hydrogels for Drug Delivery of 5-FluorouracilIranian Journal of Chemistry and Chemical Engineering, 40(5): 1386-1394 (2021).
[73] Sagoo, M.K., Gnudi, L., Diabetic Nephropathy: an OverviewDiabetic Nephropathy: Methods and Protocols, 3-7 (2020).
[74] Chen, Y., Li, Y., Shen, W., Li, K., Yu, L., Chen, Q., Ding, J., Controlled Release of Liraglutide Using Thermogelling Polymers in Treatment of DiabetesScientific reports, 6(1): 31593 (2016).
[75] Lee, A.J., Lee, Y.J., Jeon, H.Y., Kim, M., Han, E.T., Park, W.S., Hong, S.H., Kim, Y.M., Ha, K.S., Application of Elastin-Like Biopolymer-Conjugated C-Peptide Hydrogel for Systemic Long-Term Delivery Against Diabetic Aortic DysfunctionActa Biomaterialia, 118: 32-43 (2020).
[76] Kim, Y.C., Shin, M.D., Hackett, S.F., Hsueh, H.T., Lima e Silva, R., Date, A., Han, H., Kim, B.J., Xiao, A., Kim, Y., Ogunnaike, L., Gelling Hypotonic Polymer Solution for Extended Topical Drug Delivery to the EyeNature biomedical engineering, 4(11): 1053-1062 (2020).
[78] Pacelli, S., Acosta, F., Chakravarti, A.R., Samanta, S.G., Whitlow, J., Modaresi, S., Ahmed, R.P., Rajasingh, J., Paul, A., Nanodiamond-Based Injectable Hydrogel for Sustained Growth Factor Release: Preparation, Characterization and in Vitro AnalysisActa biomaterialia, 58: 479-491 (2017).
 [79] Xue, K., Zhao, X., Zhang, Z., Qiu, B., Tan, Q.S.W., Ong, K.H., Liu, Z., Parikh, B.H., Barathi, V.A., Yu, W., Wang, X., Sustained Delivery of Anti-VEGFs from Thermogel Depots Inhibits Angiogenesis Without the Need for Multiple InjectionsBiomaterials science, 7(11): 4603-4614 (2019).
[80] Cao, D., Chen, X., Cao, F., Guo, W., Tang, J., Cai, C., Cui, S., Yang, X., Yu, L., Su, Y., Ding, J., An Intelligent Transdermal Formulation of ALA‐Loaded Copolymer Thermogel with Spontaneous Asymmetry by Using Temperature‐Induced Sol–Gel Transition and Gel–Sol (Suspension) Transition on Different SidesAdvanced Functional Materials, 31(22): 2100349 (2021).
[81] Chen, Y., Tang, Y., Zhang, Y.C., Huang, X.H., Xie, Y.Q., Xiang, Y., A Metabolomic Study of Rats with Doxorubicin-Induced Cardiomyopathy and Shengmai Injection TreatmentPLoS One, 10(5): e0125209 (2015).
[82] Sharifi-Rad, J., Quispe, C., Patra, J.K., Singh, Y.D., Panda, M.K., Das, G., Adetunji, C.O., Michael, O.S., Sytar, O., Polito, L., Živković, J., Paclitaxel: Application in Modern Oncology and Nanomedicine-Based Cancer TherapyOxidative medicine and cellular longevity, (2021).
[83] Liu, M., Song, X., Wen, Y., Zhu, J.L., Li, J., Injectable Thermoresponsive Hydrogel Formed by Alginate-G-Poly (N-Isopropylacrylamide) That Releases Doxorubicin-Encapsulated Micelles as a Smart Drug Delivery SystemACS applied materials & interfaces, 9(41): 35673-35682 (2017).
[84] Wu, Y.L., Wang, H., Qiu, Y.K., Liow, S.S., Li, Z., Loh, X.J., PHB‐Based Gels as Delivery Agents of Chemotherapeutics for the Effective Shrinkage of TumorsAdvanced healthcare materials, 5(20): 2679-2685 (2016).
[85] Shao, J., Ruan, C., Xie, H., Li, Z., Wang, H., Chu, P.K., Yu, X.F., Black‐Phosphorus‐Incorporated Hydrogel as a Sprayable and Biodegradable Photothermal Platform for Postsurgical Treatment of CancerAdvanced Science, 5(5): 1700848 (2018).
[86] Liow, S.S., Karim, A.A., Loh, X.J., Biodegradable Thermogelling Polymers for Biomedical ApplicationsMRS Bulletin, 41(7): 557-566 (2016).
[87] Huang, Q., Zou, Y., Arno, M.C., Chen, S., Wang, T., Gao, J., Dove, A.P., Du, J., Hydrogel Scaffolds for Differentiation of Adipose-Derived Stem CellsChemical Society Reviews, 46(20): 6255-6275 (2017).
[88] Sepehrianazar, A., Hydrogels and Their Novel ApplicationsIranian Journal of Chemistry and Chemical Engineering, 42(4): 1099-1110 (2023).
[89] Baei, P., Jalili-Firoozinezhad, S., Rajabi-Zeleti, S., Tafazzoli-Shadpour, M., Baharvand, H., Aghdami, N., Electrically Conductive Gold Nanoparticle-Chitosan Thermosensitive Hydrogels for Cardiac Tissue EngineeringMaterials Science and Engineering: C, 63: 131-141 (2016).
[90] Fan, Z., Xu, Z., Niu, H., Gao, N., Guan, Y., Li, C., Dang, Y., Cui, X., Liu, X.L., Duan, Y., Li, H., An Injectable Oxygen Release System to Augment Cell Survival and Promote Cardiac Repair Following Myocardial InfarctionScientific reports, 8(1): 1371 (2018).
[91] Li, R., Li, Y., Wu, Y., Zhao, Y., Chen, H., Yuan, Y., Xu, K., Zhang, H., Lu, Y., Wang, J., Li, X., Heparin-Poloxamer Thermosensitive Hydrogel Loaded with bFGF and NGF Enhances Peripheral Nerve Regeneration in Diabetic RatsBiomaterials, 168: 24-37 (2018).
[92] Zhao, Y.Z., Jiang, X., Lin, Q., Xu, H.L., Huang, Y.D., Lu, C.T., Cai, J., Thermosensitive Heparin‐Poloxamer Hydrogels Enhance the Effects of GDNF on Neuronal Circuit Remodeling and Neuroprotection After Spinal Cord InjuryJournal of Biomedical Materials Research Part A, 105(10): 2816-2829 (2017).
[93] Liu, M., Zeng, X., Ma, C., Yi, H., Ali, Z., Mou, X., Li, S., Deng, Y., He, N., Injectable Hydrogels for Cartilage and Bone Tissue EngineeringBone research, 5(1): 1-20 (2017).
[94] Zhang, J., Zhang, M., Lin, R., Yun, S., Du, Y., Wang, L., Yao, Q., Zannettino, A., Zhang, H., Allogeneic Primary Mesenchymal Stem/Stromal Cell Aggregates Within Poly (N-Isopropylacrylamide-Co-Acrylic Acid) Hydrogel for Osteochondral RegenerationApplied Materials Today, 18: 100487 (2020).
[95] Zhang, Y., Zhang, J., Chang, F., Xu, W., Ding, J., Repair of Full-Thickness Articular Cartilage Defect Using Stem Cell-Encapsulated ThermogelMaterials Science and Engineering: C, 88: 79-87 (2018).
[96] Dong, L., Wang, S.J., Zhao, X.R., Zhu, Y.F., Yu, J.K., 3D-Printed Poly (ε-Caprolactone) Scaffold Integrated with Cell-Laden Chitosan Hydrogels for Bone Tissue EngineeringScientific reports, 7(1): 13412 (2017).
[97] Suntornnond, R., An, J., Chua, C.K., Bioprinting of Thermoresponsive Hydrogels for Next Generation Tissue Engineering: A ReviewMacromolecular Materials and Engineering, 302(1): 1600266 (2017).
[98] Roehm, K.D., Madihally, S.V., Bioprinted Chitosan-Gelatin Thermosensitive Hydrogels Using an Inexpensive 3D PrinterBiofabrication, 10(1): 015002 (2017).
[99] Schulz, A., Wahl, S., Rickmann, A., Ludwig, J., Stanzel, B.V., von Briesen, H., Szurman, P., Age-Related Loss of Human Vitreal ViscoelasticityTranslational vision science & technology, 8(3): 56-56 (2019).
[100] Lin, Q., Lim, J.Y., Xue, K., Su, X., Loh, X.J., Polymeric Hydrogels as a Vitreous Replacement Strategy in the EyeBiomaterials, 268: 120547 (2021).
[101] Liu, Z., Liow, S.S., Lai, S.L., Alli-Shaik, A., Holder, G.E., Parikh, B.H., Krishnakumar, S., Li, Z., Tan, M.J., Gunaratne, J., Barathi, V.A., Retinal-Detachment Repair and Vitreous-Like-Body Reformation Via a Thermogelling Polymer EndotamponadeNature biomedical engineering, 3(8): 598-610 (2019).
[102] Fan, R., Cheng, Y., Wang, R., Zhang, T., Zhang, H., Li, J., Song, S. , Zheng, A., Thermosensitive Hydrogels and Advances in their Application in Disease TherapyPolymers, 14(12): 2379 (2022).
[103] Huang, L., Li, M., Li, H., Yang, C., Cai, X., Study of Differential Properties of Fibrochondrocytes and Hyaline Chondrocytes in Growing RabbitsBritish Journal of Oral and Maxillofacial Surgery, 53(2): 187-193 (2015).
[104] Yang, C., Tibbitt, M.W., Basta, L., Anseth, K.S., Mechanical Memory and Dosing Influence Stem Cell FateNature materials, 13(6): 645-652 (2014).
[105] Ko, D.Y., Patel, M., Lee, H.J., Jeong, B., Coordinating Thermogel for Stem Cell Spheroids and Their Cyto‐EffectivenessAdvanced Functional Materials, 28(7): 1706286 (2018).
[107] Kim, S.J., Park, M.H., Moon, H.J., Park, J.H., Ko, D.Y., Jeong, B., Polypeptide Thermogels as a Three Dimensional Culture Scaffold for Hepatogenic Differentiation of Human Tonsil-Derived Mesenchymal Stem CellsACS applied materials & interfaces, 6(19): 17034-17043 (2014).
[108] Abraham, A.A., Means, A.K ., Clubb Jr, F.J., Fei, R., Locke, A.K., Gacasan, E.G., Coté, G.L., Grunlan, M.A., Foreign Body Reaction to a Subcutaneously Implanted Self-Cleaning, Thermoresponsive Hydrogel Membrane for Glucose BiosensorsACS biomaterials science & engineering, 4(12): 4104 – 4111 (2018).
[109] Means, A.K., Dong, P., Clubb, F.J., Friedemann, M.C., Colvin, L.E., Shrode, C.A., Coté, G.L., Grunlan, M.A., A Self-Cleaning, Mechanically Robust Membrane for Minimizing the Foreign Body Reaction: Towards Extending the Lifetime of Sub-Q Glucose BiosensorsJournal of Materials Science: Materials in Medicine, 30: 1-11 (2019).
[110] El-Husseiny, H.M., Mady, E.A., Hamabe, L., Abugomaa, A., Shimada, K., Yoshida, T., Tanaka, T., Yokoi, A., Elbadawy, M., Tanaka, R., Smart/Stimuli-Responsive Hydrogels: Cutting-Edge Platforms for Tissue Engineering and Other Biomedical ApplicationsMaterials Today Bio, 13: 100186 (2022).