Nashrieh Shimi va Mohandesi Shimi Iran

Nashrieh Shimi va Mohandesi Shimi Iran

Polycaprolactone Nanocapsules Containing Olaparib as a Sustained-Release Drug Delivery System for Breast Cancer Treatment

Document Type : Research Article

Authors
Maryam Shojaeifard 1
Noorhan Jasim Mohammed Al-Juaifari 1
Zohreh Mirjafary 1
Mohamad Hasan Jafari Sayadi 2
Hamid Saeidian 3
1 Department of Chemistry, Science and Research Branch, Islamic Azad University, Tehran, I.R.IRAN
2 Faculty of Agriculture and Natural Resources, Payam Noor University, Tehran, I.R.IRAN
3 Faculty of Basic Sciences, Payam Noor University, Tehran, I.R.IRAN
Abstract
Breast cancer is the most prevalent type of cancer in women. Olaparib (OLB) is an approved drug for treating this specific cancer; however, due to its limited water solubility and high dosage requirements, an effective drug delivery system (DDS) is essential. In this study, a novel and efficient DDS for OLB was developed using polycaprolactone (PCL) nanocapsules, and its physical and chemical properties were evaluated over a 40-day period. The nanocapsules achieved a drug loading efficiency of 91%. They exhibited an average particle size of 221 nm, a polydispersity index of 0.196, and a zeta potential of -29.70 mV, indicating the complete stability of the OLB@PCL nanocapsules. TEM images revealed a spherical morphology without any signs of aggregation. Furthermore, after 40 days of storage at room temperature, the nanocapsules remained stable, showing no signs of instability, aggregation, or decomposition. In vitro release profile studies demonstrated the controlled release of OLB from the PCL nanocapsules. Finally, the MTT assay revealed that the OLB@PCL nanocapsules exhibited greater cytotoxic activity against MCF-7 cell lines compared to free OLB, with an IC50 value of 522 μg/mL versus 703 μg/mL, respectively.
Keywords
Polycaprolactone nanocapsules
Olaparib
Drug delivery system
MTT assay
Polymeric nanoparticles
Subjects
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