Nashrieh Shimi va Mohandesi Shimi Iran

Nashrieh Shimi va Mohandesi Shimi Iran

Microstructural Study of Poly(Vinyl Alcohol) Nano-Fiberous Bio-Scaffold from Global Data

Document Type : Research Article

Authors
Mina Farokhzadeh 1
Hajir Kourki 2
Dariush Afzali 3
Sina Modiri 2
1 Biotechnology Group, Chemistry and Chemical Engineering Department, Graduate University of Advanced Technology, Kerman, I.R. IRAN
2 Polymer Engineering Group, Chemistry and Chemical Engineering Department, Graduate University of Advanced Technology, Kerman, I.R. IRAN
3 Chemistry Group, Chemistry and Chemical Engineering Department, Graduate University of Advanced Technology, Kerman, I.R. IRAN
Abstract
For nano-fibrous bio-scaffolds like wound dresses, control of microstructural factors such as fiber diameter, number, and size of cells is important. In this study, the microstructure is investigated from data from BET and density tests. The voltage of the electrospinning process is one of the most important parameters for the control of the microstructure. To study a wider voltage range, the existing electrospinning device is upgraded to 70 kV. In this research, the samples are spun from two types of polymers of different molecular weights in the range of 20 to 40 kV. For increasing biocompatibility and biodegradability, the polymers are purified with the phase separation method. This purification is investigated with FT-IR and melting point tests. The result of the study of the microstructure shows that, as the electrospinning voltage increases, firstly, the diameter of the fiber increases, the number of cells decreases, and the size of the cell increases. Then, the diameter of the fibers  decreased, the number of cells increased and the size of the cells decreased. In most electrospinning voltages, the mats produced from higher molecular weight polymer have a smaller fiber, a larger cell number, and a smaller cell size. But in the voltage of 25 and 40 kV, the mats have inverse results. The FT-IR and melting point results show that the phase separation method is effective for purification. Finally, it can be said that the effect of the voltage on the microstructure parameter up to 30 kV is different thereafter.
Keywords
Nano-fiber
Microstructure
Cell size
Cell number
Global data

Subjects

[1] Elsner J.J., Shefy-Peleg A ., Zilberman M., Novel Biodegradable Composite Wound Dressings With Controlled Release of Antibiotics: Microstructure, Mechanical and Physical Properties, Biomedical Materials Research Part B, 93: 425-435 (2010).
[2] Mele E., Electrospinning of Natural Polymers for Advanced Wound Care: Towards Responsive and Adaptive Dressings, Materials Chemistry B, 4: 4801-4812  (2016).
[3] Dong R.-H., Jia Y.-X., Qin C.-C., Zhan L., Yan X., Cui L., Zhou Y., Xingyu., Long J.Y.-Z.,  In-Situ Deposition of Personalized Nanofibrous Dressing via a Handy Electrospinning Device for Skin Wound Care, Nanoscale, 8: 3482-3488 (2016).
[4] Sewall G.K., Robertson K.M., Connor N.P., Heisey D.M., Hartig G.K., Effect of Topical Mitomycin on Skin Wound Contraction, Arch Facial Plast Surg, 5: 59-62 (2003).
[5] Gholipour-Kanani A., Bahrami S.H., Joghataie M.T., Samadikuchaksaraei A., Nanofibrous Scaffolds based on Poly(caprolactone)/Chitosan/ Poly(vinyl alcohol) Blend for Skin Tissue Engineering, Polymer Science and Technology, 26: 159-170 (2013).
[6] Aruana N.M., Sriyantia I., Edikresnhaa D., Suciatic T., Munira M.M ., Polyvinyl Alcohol/Soursop Leaves Extract Composite Nanofibers Synthesized Using Electrospinning Technique and Their Potential as Antibacterial Wound Dressing, Procedia Engineering, 170: 31-35 (2017).
[7] Basar A.O., Castro S., Torres-Giner S., Lagaron J.M., Sasmazel H.T., Novel Poly(ε-caprolactone)/Gelatin Wound Dressings Prepared by Emulsion Electrospinning with Controlled Release Capacity of  Ketoprofen Anti-inflammatory Drug, Materials for Biological Applications, 81: 459-468 (2017).
[8] Senthamizhan A., Balusamy B., Uyar T., Glucose Sensors Based on Electrospun Nanofibers: A Review, Analytical and Bioanalytical Chemistry, 408: 1285-1306 (2016).
[9] Andreas G., Wendorff J.H., Electrospinning: A Fascinating Method for the Preparation of Ultrathin Fibers, Angewandte Chemie (International ed. in English), 46: 5670-5703 (2007).
[10] Bogdan C., Radu C., Roxana S., Rozemarie M.L., G L.I ., Oana C., Fiber Diameter in Electrospinning Process, Journal of Electrostatics, 71: 189-198 (2013).
[11] Xiaoxia Sun., Fujimoto T ., Uyama H., Fabrication of A poly(vinyl alcohol) Monolith via Thermally Impacted Non-Solvent-Induced Phase Separation, Polymer Journal, 45: 1101-1106 (2013).
[12] Mansur HS ., Sadahira CM., Souza AN ., Mansur AAP., FTIR Spectroscopy Characterization of Poly (vinyl alcohol) Hydrogel with Different Hydrolysis Degree and Chemically Crosslinked with Glutaraldehyde, Materials Science and Engineering C, 28: 539-548 (2008).
[13] Han Y., Rheem Y., Lee K.-H., Kim H., Myung N.V., Synthesis and Characterization of Orthorhombic-MoO3 Nanofibers with Controlled Morphology and Diameter, Industrial and Engineering Chemistry, 62: 231-238  (2018).
[14] Kaerkitcha N., Chuangchote S ., Sagawa T., Control of Physical Properties of Carbon Nanofibers Obtained from Coaxial Electrospinning of PMMA and PAN with Adjustable Inner/Outer Nozzle-Ends, Nanoscale Research Letters, 11: 111-186 (2016).
[15] Sharma A., Gupta A., Rath G., Goyal A., Mathura R.B., Dhakate S.R., Electrospun Composite Nanofiber-Based Transmucosal Patch for Anti-Diabetic Drug Delivery, Journal of Materials Chemistry B, 1: 3410-3418 (2013).
[16] Zander N.E., Strawhecker K.E., Orlicki J.A., Rawlett A.M ., Beebe T.P., Coaxial Electrospun Poly(methyl methacrylate) Polyacrylonitrile Nanofibers: Atomic Force Microscopy and Compositional Characterization, Chemistry and Biochemistry, 115: 12441-12447 (2011).
[17] Vellayappan M.V., Venugopal J.R., Ramakrishna B.S., Ray S., Ismail A.F., Mandal M., Ayyar M., Seal S., Jaganathanh S.K., Electrospinning Applications from Diagnosis to Treatment of Diabetes, RSC Advances, 6: 83638-83655 (2016).
[18] Li X., Li Z., Wang L., Ma G., Meng F., Pritchard R.H., Gill E.L., Liu Y., Huang Y.Y., Low-Voltage Continuous Electrospinning Patterning, ACS Applied Materials & Interfaces, 8: 32120-32131  (2016).
[19] Kwon G.-W., Gupta K.C., Jung K.-H ., Kang I.-K., Lamination of Microfibrous PLGA Fabric by Electrospinning a Layer of Collagenhydroxyapatite Composite Nanofibers for Bone Tissue Engineering, Biomaterials Research, 21: 11-21 (2017).
[20] Geng X., Kwon O-H ., Jang J., Electrospinning of Chitosan Dissolved in Concentrated Acetic Acid Solution, Biomaterials Research, 26: 5427–5432 (2005).
[21] Ding Y., Zhang P., Jiang Y., Xu F., Yin J ., Zuo Y., Mechanical Properties of Nylon-6/SiO2 Nanofibers Prepared by Electrospinning, Materials Letters, 63: 34-36  (2009).
[22] Jinmei D., Samantha S ., Xiangwu Z., Diameter Control of Electrospun Polyacrylonitrile/Iron Acetylacetonate Ultrafine Nanofibers, Journal of Polymer Science Part B: Polymer Physics, 46: 1611-1618 (2008).
[23] Rajan Unnithan A., Nejad A., Kurup Sasikala A.R., George Thomas R., YeonJeong Y., Dongmei Wu., Hee Park C., Sang Kim C., Electrospun Zwitterionic Nanofibers with in Situ Decelerated Epithelialization Property for Non-Adherent and Easy Removable Wound Dressing Application, Chemical Engineering Journal, 287: 640-648  (2016).