Nashrieh Shimi va Mohandesi Shimi Iran

Nashrieh Shimi va Mohandesi Shimi Iran

In-situ Separation of Succinic Acid from Fermentation Broth of Corynebacterium Glutamicum

Document Type : Research Article

Authors
Sayed Abbas Shojaosadati
Parisa Chardavali
Department of Chemical Engineering and Biotechnology, Faculty of Chemical Engineering, Tarbiat Modares University, Tehran, I.R. IRAN
Abstract
The microbial production of chemicals through fermentation is often limited by product inhibition during the fermentation process. To overcome this challenge, several strategies have been proposed, including environmental condition optimization, additive supplementation, culture medium reformulation, and strain engineering. In the anaerobic fermentation of succinic acid using Corynebacterium glutamicum, the accumulation of succinic acid in the culture broth significantly reduces production yield. Therefore, this study explores the potential of improving yield through in-situ product removal techniques. A diverse set of solvents, including plant-based oils, alcohols, and alkanes, was evaluated based on biocompatibility, solubility, economic viability, and other critical parameters relevant to in-situ extraction. Aspen Plus software was used to simulate and screen potential solvents for effective succinic acid separation. Among the candidates, octanol, octane, and oleyl alcohol were further assessed for biocompatibility. Experimental findings revealed that glucose consumption by C. glutamicum was significantly inhibited after 24 hours of fermentation, indicating the optimal time point for solvent addition. Oleyl alcohol emerged as the most effective solvent due to its excellent biocompatibility, high affinity for succinic acid, and easy recoverability via distillation. The best separation efficiency was achieved at a 10%  (v/v) solvent concentration, leading to an approximate 15%  increase in succinic acid yield.
Keywords
In-situ separation
Fermentation
Succinic acid
Corynebacterium glutamicum

Subjects

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