نانوفرمولاسیون جینجرول و بررسی اثر سایتوتوکسیک آن بر رده ی سلولی MCF-7

نوع مقاله : علمی-پژوهشی

نویسندگان

1 گروه بیوتکنولوژی، دانشکده مهندسی شیمی، دانشگاه صنعتی نوشیروانی، بابل، ایران

2 گروه فارماکولوژی، دانشکده پزشکی، دانشگاه علوم پزشکی بابل، بابل، ایران

چکیده

جینجرول­ ها و شوگول­ ها مواد موثر موجود در ریشه زنجبیل می­ باشند که خواص دارویی متعددی را به این ادویه پرکاربرد می­ بخشند. در پژوهش پیش رو، ترکیبات موثر ریشه زنجبیل استخراج و توسط ستون کروماتوگرافی خالص سازی شد. به منظور افزایش فراهمی زیستی و بهبود خواص دارویی، جینجرول حاصل در نانوذره ­های کیتوزان کپسوله شد. بازده انکپسوله شدن 80% به­ دست آمد. آنالیزهای AFM و DLS جهت تعیین اندازه­ی ذره ­ها و پراکندگی اندازه­ ی آن­ها انجام شد. اندازه­ ی متوسط نانوذره های حاصل11/21 نانومتر بود. نانوذره ­ها، کروی شکل و با الگوریتم توزیع اندازه ذره­ های مناسب حاصل شدند. در ادامه آنالیز سمیت روی سلول­ های­ سرطان سینه MCF-7 انجام پذیرفت. نتیجه­ ها حاکی از آن بود که نانوذره ­های کیتوزان حاوی جینجرول، بازدارندگی مناسبی روی رشد سلول­ های سرطانی داشته­ اند. جینجرول کپسوله شده در نانوذره­ ها با غلظت 2 میلی­ گرم بر میلی­ لیتر، سبب از بین رفتن 90 درصد از سلول­ ها طی زمان 48 ساعت شد.

کلیدواژه‌ها

موضوعات

مراجع

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