Nashrieh Shimi va Mohandesi Shimi Iran

Nashrieh Shimi va Mohandesi Shimi Iran

Investigation of Light Stress Effect on Beta-Carotene Storage in Pure and Mixed Cultures of Microalgae

Document Type : Research Article

Authors
Azita Gorbani 1
Maryam Hosseini 2
Sirous Ebrahimi 1
1 Biotechnology Research Center, Sahand University of Technology, Tabriz, I.R. IRAN
2 Department of Chemical Engineering, Faculty of Engineering, Azarbaijan Shahid Madani University, Tabriz, I.R. IRAN
Abstract
β-Carotene is a strongly colored red-orange pigment. Microalgae are high of interest since they can be a great source for synthesized Beta-carotene. So far, the production of beta-carotene has been studied in pure cultures. Because of the practical problems with pure cultures, in this study, the high light intensity has been applied to mixed microalgae species obtained from the Caspian Sea and also a pure culture of Dunaliella salina and the amounts of beta-carotene, chlorophyll, and proteins have been measured. Operating conditions in the light stress were the same for both samples: pH=7.5, temperature 26-28 °C and stirring at 160 rpm. On the 6th day, the stress was applied. The final beta-carotene concentration of microalgae Dunaliella salina was increased from 5.9 to 19.5 mol Beta-Carotene/g Protein. However, the concentration was reached from 7.3 to 22.7 mol Beta-Carotene/g Protein for Caspian Sea microalgae. The initial and final concentration of protein was 467.3, 1239.9 and 495.2 and 1131.3 mg/L for pure and mixed culture, respectively. The results of this study show the potential of Caspian Sea mixed microalgae culture as a viable alternative for Dunaliella salina microalgae for beta-carotene production on industrial scales. Therefore, mixed microalgae culture can be used for Beta-carotene production which is preferable and more economical than sterile systems, and consequently, the possibility of process industrialization will be increased.
Keywords
Beta Carotene
Light Stress
Microalgae Dunaliella Salina
Caspian Sea microalgae

Subjects

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