Dispersive Liquid-Liquid Microextraction for Pre-concentration of fentanyl in Water Samples and Its Measuring Using UV-Visible Spectroscopy

Document Type : Research Article


1 Department of Chemistry, Faculty of Basic Sciences, Imam Hossein University, Tehran, I.R. IRAN

2 Faculty of Pharmacy, Baqiyatallah University of Medical Sciences, Tehran, I.R. IRAN


In the present study, Dispersive Liquid-Liquid Microextraction (DLLME) method coupled with UV-Vis spectrophotometry was used for pre-concentration and measurement of fentanyl in water, plasma, and urine samples. 10mL of water sample containing fentanyl adjusted by phosphate buffer at pH 10 and placed in a 15-millimeter- glass tube with a conical end. Then, usinga microsyringe, a chloroform solution containing aliquot 336s was rapidly injected into the solution, and then a cloudy solution was created. At this stage, the resulting product was extracted into tiny drops of chloroform. Next, the mixture was centrifuged for a specific time and the organic phase was deposited at the tube. After eliminating the upper aqueous phase, the residual organic phase was taken with a microsyringe and placed into a micro-cell of quartz and its adsorption in comparison with the control solution in the wavelengths (fentanyl 265 nm) was read. In this method, conventional dispersive solvents are not used to disperse the extracted solvent because the injectable aliquot336s, along with chloroform, other than creating cation along with ion pairs with solution, plays a role as a dispersive solvent for the formation of a cloud solution, which not only results in dispersive liquid-liquid microextraction, but also considers as a green method. The detection limit of the method to measure fentanyl was 0/002 mg/L  respectively, and the calibration curve was linear in the range of 0/01-52mg/L. The relative standard deviations(at n=7) for the extraction of fentanyl (3.5 ng/mL), was 2.7 %, and the enrichment factors was 55.5. Simplicity, being fast, low cost, and high pre-concentration factor are some of the advantages of the present method. Complex devices have been used for almost all measurements of Fentanyl. Some figures relating to the suitability of this method are comparable to those reported previously.


Main Subjects

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