Nashrieh Shimi va Mohandesi Shimi Iran

Nashrieh Shimi va Mohandesi Shimi Iran

Spectral Modification of Light Using Phycocyanin Pigment for Enhancing Growth of Green Microalgae Chlorella sp.

Document Type : Research Article

Authors
Hossein Delavari Amrei 1
Zahra Khoobkar 2
1 Department of Chemical Engineering, Faculty of Engineering, University of Bojnord, Bojnord, Iran
2 Department of Chemical Engineering, Science and Research Branch, Islamic Azad University, Tehran, I.R. IRAN
Abstract
The focus of this research is about spectral modification of light using phycocyanin solution for enhancing the growth of microalgae Chlorella sp.. For this purpose, two double-layer flat panel photobioreactor was constructed. In the front layer of the main reactor, microalgae Chlorella sp. was cultivated and the rear layer was filled with phycocyanin solution. In the control reactor (without phycocyanin), the front layer was filled with deionized water instead of phycocyanin solution. Photobioreactors were under the radiation of white LED with the intensity of 490 µmolphoton m-2/s at the temperature of 27±1ºC. Compared to the control system, results showed that biomass productivity (P) and maximum specific growth rate (µmax) increased 75% and 90%, respectively. Also, the content of chlorophyll a was increased, significantly. But lipid content of cells decreased under modified light using phycocyanin.
Keywords
Microalgae
Photobioreactor
Phycocyanin
Chlorella
Spectral modification

Subjects

 [1] Chisti Y., Biodiesel from Microalgae. Biotechnology Advances, 25(3): 294-306 (2007).
 [2] de Godos, I., Blanco, S., García-Encina, P.A., Becares, E., Muñoz, R., Influence of Flue Gas Sparging on the Performance of High Rate Algae Ponds Treating Agro-Industrial Wastewaters, Journal of Hazardous Materials, 179(1): 1049-1054 (2010).
[3] Ho, S.H., Chen, C.Y., Lee, D.J., Chang, J.S., Perspectives on Microalgal CO2-Emission Mitigation Systems - A Review. Biotechnology Advances, 29(2): 189-198 (2011).
[4] Raes, E.J., Isdepsky, A., Muylaert, K., Borowitzka, M.A., Moheimani, N.R., Comparison of Growth of Tetraselmis in a Tubular Photobioreactor (Biocoil) and a Raceway Pond. Journal of Applied Phycology, 26(1): 247-255 (2014).
[5] Sidler W.A., "Phycobilisome and Phycobiliprotein Structures, in the Molecular Biology of Cyanobacteria”, Bryant D.A. (Editor). Springer Netherlands: Dordrecht. 139-216 (1994).
[6] Chrismadha T., Borowitzka M. A., Effect of Cell Density and Irradiance on Growth, Proximate Composition and Eicosapentaenoic Acid Production of Phaeodactylum Pricornutum Grown in a Tubular Photobioreactor. Journal of Applied Phycology, 6(1): 67-74 (1994).
[7] Danesi, E.D.G., Rangel-Yagui, C.O., Carvalho, J.C.M., Sato, S., Effect of Reducing the Light Intensity on the Growth and Production of Chlorophyll by Spirulina Platensis. Biomass and Bioenergy, 26(4): 329-335 (2004).
[8] Matthijs, H.C., Balke, H., Van Hes, U.M., Kroon, B.M., Mur, L.R., Binot, R.A., Application of Light‐Emitting Diodes in Bioreactors: Flashing Light Effects and Energy Economy in Algal Culture (Chlorella Pyrenoidosa). Biotechnology and Bioengineering, 50(1): 98-107 (1996).
[9] Wang C.-Y., Fu C.-C., Liu Y.-C., Effects of Using Light-Emitting Diodes on the Cultivation of Spirulina Platensis. Biochemical Engineering Journal, 37(1): 21-25 (2007).
[10] Choul‐Gyun L., P.B. Ø., High‐Density Algal Photobioreactors Using Light‐Emitting Diodes. Biotechnology and Bioengineering, 44(10): 1161-1167 (1994).
[11] Kohen E., Santus R., Hirschberg G.G., “Photobiology". Academic Press, New York (1995).
[12] Klampaftis, E., Ross, D., McIntosh, K.R., Richards, B.S.,  Enhancing the Performance of Solar Cells via Luminescent Down-Shifting of the Incident Spectrum: A Review. Solar Energy Materials and Solar Cells, 93(8): 1182-1194 (2009).
[13] Strümpel, C., McCann, M., Beaucarne, G., Arkhipov, V., Slaoui, A., Švrček, V., Del Cañizo, C., Tobias, I., Modifying the Solar Spectrum to Enhance Silicon Solar Cell Efficiency—An Overview of Available Materials. Solar Energy Materials and Solar Cells, 91(4): 238-249 (2007).
[14] Amrei, H.D., Nasernejad, B., Ranjbar, R., Rastegar, S.,  Spectral Shifting of UV-A Wavelengths to Blue Light for Enhancing Growth Rate of Cyanobacteria. Journal of Applied Phycology, 26(3): 1493-1500 (2014).
[15] Delavari Amrei H, Ranjbar R., Influence of Fluorescent Coating at Rear and front Side of a Flat Panel Photobioreactor on Algal Growth. Journal of Applied Phycology, 30(2): 901-907 (2018).
[16] Amrei H.D., Ranjbar R., Rastegar S., Nasernejad B., Using Fluorescent Material for Enhancing Microalgae Growth Rate in Photobioreactors. Journal of Applied Phycology, 27(1): 67-74 (2015).
[17] Delavari Amrei, H., Nasernejad, B., Ranjbar, R., Rastegar, S., An Integrated Wavelength-Shifting Strategy for Enhancement of Microalgal Growth Rate in PMMA-and Polycarbonate-Based Photobioreactors. European Journal Phycology, 49(3): 324-331 (2014).
[18] Mohsenpour S.F., Willoughby N., Effect of CO2 Aeration on Cultivation of Microalgae in Luminescent Photobioreactors. Biomass and Bioenergy, 85: 168-177 (2016).
[19] Wondraczek, L., Batentschuk, M., Schmidt, M.A., Borchardt, R., Scheiner, S., Seemann, B., Schweizer, P., Brabec, C.J., Solar Spectral Conversion for Improving the Photosynthetic Activity in algae reactors. Nature Communications, 4: 2047 (2013).
[20] Wondraczek, L., Tyystjärvi, E., Méndez‐Ramos, J., Müller, F.A., Zhang, Q.,  Shifting the Sun: Solar Spectral Conversion and Extrinsic Sensitization in Natural and Artificial Photosynthesis. Advanced Science, 2(12): 1500 (2015).
[21] Sung, M.G., Han, J.I., Lee, B., Chang, Y.K., Wavelength Shift Strategy to Enhance Lipid Productivity of Nannochloropsis Gaditana. Biotechnology for Biofuels, Biotechnology for Biofuels., 11(1): 70 (2018).
[22] Esfahani, R.H., Shafii, M.B., Roshandel, R., Energy Efficient Cultivation of Microalgae Using Phosphorescence Materials and Mirrors. Sustainable Cities and Societ., 41: 449-454 (2018).
[23] Zou, L., Gu, Z., Zhang, N., Zhang, Y., Fang, Z., Zhu, W., Zhong, X., Ultrafast Synthesis of Highly Luminescent Green- to Near Infrared-Emitting CdTe Nanocrystals in Aqueous Phase. Journal of Materials Chemistry, 18(24): 2807-2815 (2008).
[24] Bligh E.G., Dyer W.J., A Rapid Method of Total Lipid Extraction and Purification. Canadian Journal of Biochemistry and Physiology, 37(8): 911-917 (1959).
[25] Seo, Y.H., Cho, C., Lee, J.Y., Han, J.I., Enhancement of Growth and Lipid Production from Microalgae using Fluorescent Paint Under the Solar Radiation. Bioresource Technology, 173: 193-197 (2014).
[26] Zhang, Y.M., Chen, H., He, C.L., Wang, Q.,  Nitrogen Starvation Induced Oxidative Stress in an Oil-Producing Green Alga Chlorella Sorokiniana C3. PLoS ONE, 8(7): e69225 (2013).