Investigating the Parameters Effect on Ascorbic Acid Extraction Using Sulfate Salts and Polyethylene Glycol in Aqueous Two-Phase Systems

Document Type : Research Article

Authors

1 Department of Chemical Engineering, Faculty of Engineering, University of Guilan, Rasht, I.R. IRAN

2 Department of Chemical Engineering, North Tehran Branch, Islamic Azad University, Tehran, I.R. IRAN

Abstract

Aqueous two-phase systems provide a suitable environment for the separation of biomolecules, because in such systems there is sufficient water at all stages of the process, and on the other hand, the purpose is to isolate biomolecules and maintain their activity. The purpose of this study was to investigate the effective parameters on the extraction of Ascorbic acid with Sulfate salts and Polyethylene glycol polymer in aqueous two-phase systems. The effect of molecular weight of polyethylene glycol (4000 and 8000 g / mol), salts (MnSO4, Na2SO4, MgSO4), salt concentration, and pH effect on the formation of the two-phase system at temperatures of 32, 37, and 42 °C as investigated. The results showed that by increasing the molecular weight of the polymer, the coefficient of isolation and ascorbic acid extraction percentage decreased. Also, increasing the concentration of salt and temperature, the coefficient of isolation, and the percentage of extraction of Ascorbic acid increases. The separation of Ascorbic acid largely depends on the type of salt. MnSO4 salt showed a higher extraction percent (37.4%) than two other salts. The distribution coefficient of Ascorbic acid was found to be higher at (pH = 5), and the maximum Ascorbic acid distribution coefficient for the best salt (MnSO4) was obtained at 0/423.

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References

 
[1] “Safety (MSDS) Data for Ascorbic Acid”, Oxford University., Retrieved 2007-02-21(2005).
[2] Treybal, R. E., “Mass Transfer Operation, Liquid-liquid Extraction”. Mc Graw-Hill Company, New York, (1951).
[3] Ghanadzadeh Gilani HKhayati G., Safari Keyvani, Z., Extraction of Cu(II) Ions from Aqueous Media Using PEG/Sulphate Salt Aqueous Two-Phase System, Separation Science and Technology, 51(4): (2016).
[4] Rozen, V.M., Safiulina, A.M., Shkinev K., Problems of Phase Formation in the Polyethylene Glycol-Inorganic Salt Water Systems at Metal Extraction: I. Selected Anions Including Phase Separation, Radiochemistry, 43(6): 558-561. (2001)
[5] da Silva, L.M., Meirelles, A.J.A., PEG+Potassium Phosphate+Urea Aqueous Two-Phase Systems: Phase Equilibrium and Protein Partitioning, J. Chem. Eng. Data 46: 251 (2001)
[6] Osseo – Asare, X., Zeng. K., Partition of Pyrite in Aqueous Biphasic Systems, Int. J. Miner Process, 58: 319-330. (2000)
[7] Hynes, C.A., Blanch, H.W., Prauznits, J.M., Thermodynamic Properties of Aqueous Two-Phase Polymer Systems Containing Salts and Proteins, Fluid Phase Equilib.53: 463-478. (1989)
[8] Tintinger, R., Zhu, J., Grossmann, C., Maurer, G., Partitioning of Some Amino Acids and Low Molecular Mass Peptids an Aqueous Two-Phase Systems of Polyethylene Glycol and Dextran in the Presence of Small Amounts of K2HPO4/KH2PO4-Buffer at 293K: Experimental Results and Correlation, J. Chem. Eng. Data. 42: 975. (1997)
[9] قنادزاده گیلانی، حسین.، حسین پور، هلن، “بررسی اثر نمک‌های سولفات در استخراج اسید آسکوربیک با استفاده از سامانه‌های دو فازی آبی”، پایان نامه کارشناسی ارشد، دانشگاه آزاد تهران واحد شمال. (1395)
[10] قنادزاده گیلانی، حسین.، معصومی، حدیثه.، “بررسی اثر نمک‌های فسفات در استخراج اسید مالیک با استفاده از سامانه‌های دو فازی آبی”، پایان نامه کارشناسی ارشد، دانشگاه گیلان. (1397)
[11] Porto T. S., Marques P. P., Porto C. S., Moreira, K. A., Extraction of Ascorbate Oxidase from Cucurbita maxima by Continuous Process in Perforated Rotating Disc Contactor Using Aqueous Two-Phase Systems, Appl. Biochem. Biotechnol. 160:1057-1064. (2010)
 [12] da Costa, A.R., dos Reis Coimbra, J.S ., Ferreira , L.A., Mrcos, J.C., Santoc, I.J.B., Saldana, M.D., Teixeria J.A.C., Partitioning of Bovine Lactoferrin in Aqueous Two-Phase System Containing Polyethylene Glycol and Sodium Citrate. Food and Bioproducts Processing, 95: 118-124 (2015).
[13] SE, R.A.G., Aznar, M., Prediction of Phase Equilibrium of Aqueous two Phase Systems Water + Poly (Ethylene Glycol)+ Salt. Latin American Applied Research, 31: 427-432 (2001).
[14] Albertsson, P.A., “Partition of Cell Particles and Macromolecules”, Wiley Interscience, (1986).
[15] Hart, R.A., Ogez, J.R., Builder, S.E., Use of Multifactorial Analysis to Develop Aqueous Two-Phase Systems for Isolation of Non- Native IGF-1. Bioseparations, 5: 113-121, (1995).                              
[16] Osseo – Asare, X., Zeng K., Partition of Pyrite in Aqueous Biphasic Systems, Int.J.Miner Process, 58: 319-330. (2000)
[17] Liu, W., Gaob, X., Tang, Y., Wang, R., On the Extraction and Separation of Iodide Complex of Cadmium(II) in Propyl-Alcohol Ammonium Sulfate Aqueous Biphasic System, Separation and Purification Technology, 50: 263-266 (2006)
[18] Albertsson, P.K., Tjerneld, F., Walter, H., Johnsson, G., Aqueous Two-Phase Systems, Methods Enzymol, Academic Press. San Diego, CA 228, 11. (1994)
[19] Huddleston, J.G., Ottomar, K.W., Ngonyani, D.M., Lyddiat, A., Influences of Systems and Molecular Parameters upon Fraction of Intracellular Proteins from Saccharomyces by Aqueous Two-Phase Partition.Enzyme Microb. Technol.13: 24-32. (1991)
[20] Raja, S., Ramachandra Murty, V., Thivaharan, V., Rajasekar, V., Aqueous Two Phase Systems for the Recovery of Biomolecules; A Review, Science and Technology, 1: 7-16 (2011).
[21] David, S., Soane, D.S., Polymer Applications for Biotechnology, Prentice Hall, 204-240. (1992)
[22] Zaslavsky, B.Y., “Aqueous Two-Phase Partitioning: Physical Chemistry and Bioanalytical Applications”. Marcel Dekker Inc., New York (1992)
[23] Macros, J.C., Fonseca, L.P., Ramalho, M.T., Cabral, J.M.S., Partial Purification of Penicillin Acylase from Escherichia Coli in (Polyethylene Glycol)- Sodium Citrate Aqueous Two-Phase Systems, J. Chem. B., 734: 15-22 (1999)
[24] Anathha Padmanabhhan, K.P., Goddard, E.D., Aqueous Biphase Foundation in Polyethylene Oxide- Inorganic Salt Systems, Langmuir, 3: 25. (1987)
[25] Laurence, R., Weartherley, I., “Engineering Process for Bioseparation”, Butterworth- Heinemann Ltd. (1994)
Nashrieh Shimi va Mohandesi Shimi Iran
Volume 39, Issue 3 - Serial Number 97
September 2020
Pages 163-170

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