Biodiesel Production from Iranian Native Microalgae Biomass

Shahi, Taher; Beheshti, Babak; Zonouzi, Ali; Almasi, Morteza

Biodiesel Production from Iranian Native Microalgae Biomass

Document Type : Research Article

Authors

¹ Department of Agriculture Machinery, Science And Research Branch, Islamic Azad University, Tehran, I.R. IRAN

² Iranian Research Organization for Science and Technology (IROST), I.R. IRAN

³ Department of Agriculture Machinery, Science and Research Branch, Islamic Azad University, Tehran, I.R. IRAN

Abstract

The production of renewable fuels and the gradual replacement of fossil fuels have been one of the main challenges of researchers in recent decades. Biodiesel is one of the fuels that is used by various sources and used in diesel engines. The production of biodiesel from third-generation sources has faced many challenges, especially in recent years. In this research, biodiesel was produced from microalgae oil in two wet and dry methods of native Dunaliella Sp. of Iran, and its properties were studied. The results showed that the wet method (direct method) has a higher yield than the dry method because the biodiesel is produced directly from moist biomass. So that the maximum extraction efficiency of the oil in the wet method was 17.4% and in the dry method it was 10.2%. The best extraction yield was achieved at 60 ^◦C and 30 minutes. The duration of oil extraction in the wet method due to the removal of the drying and desalting steps is considerably shorter than in the dry method. Therefore, the wet method has a high potential for biodiesel production. The microalgae Dunaliella SP. the fatty acid profile has 14 to 20 carbons and the main portion is composed of Palmitic acid (36.62%) and Stearic acid.

Keywords

20.1001.1.10227768.1400.40.3.30.9

Main Subjects

References

[1] Asif M., Muneer T., Energy Supply, Its Demands and Security Issues for Developed and Emerging Economies. Renew. Sustain. Energ. Rev. 11: 1388-1413 (2007). doi:10.1016/j.rser.2005.12.004

[2] Kumar R., Hanumantha Rao P., Arumugam M., Lipid Extraction Methods from Microalgae: A Comprehensive Review. Frontiers in Energy Research, 2: 61 (2015).

[3] Khan S.A., Rashmi Hussain M.Z., Prasad S., Banerjee U., Prospects of Biodiesel Production from Microalgae in India. Renew. Sustain. Energ. Rev. 13: 2361-2372 (2009).

[4] Arumugam M., Agarwal A., Arya M.C., Ahmed Z. Microalgae: Renewable Source for Second Generation Biofuel. Curr.Sci. 100: 1141-1142 (2011). doi:10.1007/s00253-009-1935-6

[5] Guedes R.E., Luna A.S., Torres A.R. Operating Parameters for Bio-Oil Production in Biomass Pyrolysis: A Review. Journal of Analytical and Applied Pyrolysis, 129: 134-149. (2018).

[6] Biller P., Ross A.B. Potential Yields and Properties of Oil from the Hydrothermal Liquefaction of Microalgae with Different Biochemical Content. Bioresource Technology, 102(1): 215-225 (2011).

[7] Borowitzka M.A., Moheimani N.R., “Algae for Biofuels and Energy”, Springer Dordrecht Heidelberg New York London. (2013).

[8] Wahidin S., Idris A., Yusof N.M., Kamis N.H.H., Shaleh S.R.M. Optimization of the Ionic Liquid-Microwave Assisted One-Step Biodiesel Production Process from Wet Microalgal Biomass. Energy Conversion and Management, 171: 1397-1404. (2018).

[9] Schenk P.M., Thomas-Hall S.R., Stephens E., Marx U.C., Mussgnug J.H., Posten C Hankamer B. Second Generation Biofuels: High-Efficiency Microalgae for Biodiesel Production. Bioenergy Research, 1(1): 20-43 (2008).

[10] Dismukes G.C., Carrieri D., Bennette N., Ananyev G. M., Posewitz M. C. Aquatic Phototrophs: Efficient Alternatives to Land-Based Crops for Biofuels. Current Opinion in Biotechnology, 19(3): 235-240 (2008).

[11] Searchinger T., Heimlich R., Houghton R.A., Dong F., Elobeid A., Fabiosa J., ... & Yu T.H. Use of US Croplands for Biofuels Increases Greenhouse Gases Through Emissions from Land-Use Change. Science, 319(5867): 1238-1240 (2008).

[12] Chisti, Y. Biodiesel from Microalgae. Biotechnology Advances, 25(3): 294-306 (2007).

[13] Spolaore, P., Joannis-Cassan, C., Duran, E., Isambert, A. Commercial Applications of Microalgae. Journal of bioscience and bioengineering, 101(2): 87-96 (2006).

[14] Cantrell K.B., Ducey T., Ro K.S., Hunt P.G. Livestock Waste-to-Bioenergy Generation Opportunities. Bioresource Technology, 99(17): 7941-7953 (2008).

[15] Rodolfi L., Chini Zittelli G., Bassi N., Padovani G., Biondi N., Bonini G., Tredici M.R. Microalgae for Oil: Strain Selection, Induction of Lipid Synthesis and Outdoor Mass Cultivation in a Low‐Cost Photobioreactor. Biotechnology and Bioengineering, 102(1): 100-112 (2009).

[16] Hirano A., Ueda R., Hirayama S., Ogushi Y. CO₂ Fixation and Ethanol Production with Microalgal Photosynthesis and Intracellular Anaerobic Fermentation. Energy 22(2-3): 137-142 (1997).

[17] Qin J. Bio-Hydrocarbons from Algae - Impacts of Temperature, Light and Salinity on Algae Growth. Barton, Australia: Rural Industries Research and Development Corporation; (2005).

[18] Kapoore R., Butler T., Pandhal J., Vaidyanathan S. Microwave-Assisted Extraction for Microalgae: from Biofuels to Biorefinery. Biology, 7(1): 18 (2018).

[19] Patil P.D., Gude V.G., Mannarswamy A., Cooke P., Nirmalakhandan N., Lammers P., Deng S. Comparison of Direct Transesterification of Algal Biomass under Supercritical Methanol
and Microwave Irradiation Conditions. Fuel, 97, 822-831 (2012).

[20] Richmond A. (Ed.). “Handbook of Microalgal Culture: Biotechnology and Applied Phycology”, Oxford: Blackwell Science, Vol. 577 (2004).

[21] Zebib T., “Microalgae Grown in Photobioreactors for Mass Production of Biofuel”. Rutgers University Department of Bioenvironmental Engineering September 30 (2008)

[22] Shahi T., Zonouzi A., Beheshti B., Almasi M. Comparison of Four Lipid Extraction Methods from Microalgae Dunaliella Sp. for Biodiesel Production. Iranian Journal of Chemistry and Chemical Engineering (IJCCE), 39(4): 371-378 (2020).

[23] Bligh E.G., Dyer W.J.A., Rapid Method of Total Lipid Extraction and Purification. Canadian Journal of Biochemistry and Physiology, 37(8): 911-917 (1959).

[24] Zenouzi A., Ghobadian B., Hejazi M.A., Rahnemoon P., Harvesting of Microalgae Dunaliella Salina using Electroflocculation. JAST, 15(5) (2013)

[25] زنوزی، ع. قبادیان، ب. توکلی هشجین، ت. فیض اله نژاد، م. باقرپور، ح. تأثیر ترکیب سوخت‌های دیزل و بیودیزل حاصل از پسماند روغن‌های خوراکی در عملکرد موتور اشتعال تراکمی (CI). مجله سوخت و احتراق، 1(1): 53تا59 (1387).

[26] فیض اله نژاد، م. قبادیان، ب. توکلی هشجین، ت. باقرپور، ح. زنوزی، ع. بررسی پارامترهای مؤثر بر آبشویی سوخت بیودیزل. مجله مهندسی بیوسیستم ایران، 40(2): 147تا154 (1388).

[27] ملک زاده, عابدینی, وثوقی, رشتچیان. بررسی تجربی و مدل‌سازی ترمودینامیکی فرآیند استخراج لیپید از ریزجلبک تر. پژوهش نفت، 26(4): 153تا164 (1395).

[28] Wang L., Yang B., Du X., Yi C. Optimisation of Supercritical Fluid Extraction of Flavonoids from Pueraria Lobata. Food Chemistry, 108(2): 737-741 (2008).

[29] Ghazali Q., Yasin N.H.M. The Effect of Organic Solvent, Temperature and Mixing Time on the Production of Oil from Moringa Oleifera Seeds. In IOP Conference Series: Earth and Environmental Science, IOP Publishing. 36(1): 012053 (2016).

[30] Abdullah N., Amran, N.A., Yasin N.H.M. Algae Oil Extraction from Freshwater Microalgae Chlorella Vulgaris. Malaysian Journal of Analytical Sciences, 21(3): 735-744 (2017).

[31] Suganya T., Renganathan, S. Optimization and Kinetic Studies on Algal Oil Extraction from Marine Macroalgae Ulva Lactuca. Bioresource Technology, 107: 319-326 (2012).

[32] رئوفی, موسوی گرگری, سید لطیف. بهینه‌سازی استحصال روغن از گونه میکروجلبکی Haematococcus pluvialis با استفاده از روش سطح پاسخ و بررسی پروفایل اسید چرب آن به منظور تولید بیودیزل. نشریه علمی فیزیولوژی و بیوتکنولوژی آبزیان، 7(1): 145تا160 (1970).

[33] Sathish A., Sims R.C., Biodiesel from Mixed Culture Algae via a Wet Lipid Extraction Procedure. Bioresource Technology, 118: 643-647 (2012).

[34] Huang X., Huang Z., Wen W., Yan J., Effects of Nitrogen Supplementation of the Culture Medium on the Growth, Total Lipid Content and Fatty Acid Profiles of Three Microalgae (Tetraselmis Subcordiformis, Nannochloropsis Oculata and Pavlova Viridis). Journal of applied phycology, 25(1): 129-137 (2013).

[35] Widjaja A., Chien C.C., Ju Y.H., Study of Increasing Lipid Production from Fresh Water Microalgae Chlorella Vulgaris. Journal of the Taiwan Institute of Chemical Engineers, 40(1): 13-20 (2009).

[36] Borowitzka M.A., Moheimani N. R. (Eds.). “Algae for Biofuels and Energy”, Springer, Dordrecht, the Netherlands. 5: 133-152 (2013).

[37] Ghasemi Naghdi F., González González L.M., Chan W., Schenk P.M. Progress on Lipid Extraction from Wet Algal Biomass for Biodiesel Production. Microbial Biotechnology, 9(6): 718-726 (2016).

[38] Cao H., Zhang Z., Wu X., Miao X. Direct Biodiesel Production from Wet Microalgae Biomass of Chlorella Pyrenoidosa through in Situ Transesterification. BioMed Research International, (2013).

[39] یزدانی, خسروی رینه, احمدی, اکرم. بررسی ترکیب اسید چرب و پتانسیل تولید بیودیزل برخی ریزجلبک ها در زیستگاه های مختلف. زیست شناسی کاربردی، 31(3): 152تا160 (2018).

[40] Amaro H.M., Guedes A.C., Malcata F.X. Advances and Perspectives in using Microalgae to Produce Biodiesel. Applied Energy, 88(10): 3402-3410 (2011).

[41] Knothe G. “Designer” Biodiesel: Optimizing Fatty Ester Composition to Improve Fuel Properties. Energy & Fuels, 22(2): 1358-1364 (2008).

[42] Richter B.E., Jones B.A., Ezzell J.L., Porter N.L., Avdalovic N., Pohl C. Accelerated Solvent Extraction: a Technique for Sample Preparation. Analytical Chemistry, 68(6): 1033-1039 (1996).

[43] Canakci M., Sanli H. Biodiesel Production from Various Feedstocks and Their Effects on the Fuel Properties. Journal of Industrial Microbiology & Biotechnology, 35(5): 431-441 (2008).

[44] Kenyon C.N., Rippka R., Stanier R.Y. Fatty Acid Composition and Physiological Properties of Some Filamentous Blue-Green Algae. Archiv für Mikrobiologie, 83(3): 216-236 (1972).

[45] Li Y., Naghdi F.G., Garg S., Adarme-Vega T.C., Thurecht K.J., Ghafor W.A., ... Schenk P.M. A Comparative Study: the Impact of Different Lipid Extraction Methods on Current Microalgal Lipid Research. Microbial Cell Factories, 13(1): 14 (2014).

[46] Xiong W., Li X., Xiang J., Wu Q. High-Density Fermentation of Microalga Chlorella Protothecoides in Bioreactor for Microbio-Diesel Production. Applied Microbiology and Biotechnology, 78(1): 29-36 (2008).

[47] Nascimento I.A., Marques S.S.I., Cabanelas I.T.D., Pereira S.A., Druzian J.I., de Souza C.O., ... Nascimento M.A. Screening Microalgae Strains for Biodiesel Production: Lipid Productivity and Estimation of Fuel Quality Based on Fatty Acids Profiles as Selective criteria. Bioenergy Research, 6(1): 1-13 (2013).

[48] Talebi A.F., Mohtashami S.K., Tabatabaei M., Tohidfar M., Bagheri A., Zeinalabedini M., Bakhtiari S. Fatty Acids Profiling: A Selective Criterion for Screening Microalgae Strains for Biodiesel Production. Algal Research, 2(3): 258-267 (2013).

[49] Zhukova N.V., Aizdaicher N.A. Fatty Acid Composition of 15 Species of Marine Microalgae. Phytochemistry, 39(2): 351-356 (1995).

[50] Ramos M. J., Fernández C.M., Casas, A., Rodríguez, L., Pérez, Á. Influence of Fatty Acid Composition of Raw Materials on Biodiesel Properties. Bioresource Technology, 100(1): 261-268 (2009).

[51] Roy S.K., Karim S.M.M., Rahman Z., Aziz S., Hassan S.M.M. The Fatty Acid Composition and Properties of Oil Extracted from Cotton (Gossypium Herbaceum) Seed of Bangladesh. Bangladesh Journal of Scientific and Industrial Research, 47(3): 303-308 (2012).

[52] Chowdhury K., Banu L.A. Khan S., Latif A. Studies on the Fatty Acid Composition of Edible Oil. Bangladesh Journal of Scientific and Industrial Research, 42(3): 311-316 (2007).

[53] Al-Bachir, M., Sahloul, H. Fatty Acid Profile of Olive Oil Extracted from Irradiated and Non-Irradiated Olive Fruits. International Journal of Food Properties, 20(11): 2550-2558 (2017)

[54] Mancini, A., Imperlini, E., Nigro, E., Montagnese, C., Daniele, A., Orrù, S., Buono, P. Biological and Nutritional Properties of Palm Oil and Palmitic Acid: Effects on Health. Molecules, 20(9): 17339-17361 (2015).

[55] Varsha Thakur, Seema Paroha, Ravi Prakash Mishra. Free Fatty Acid Profile of Seven Sesame (Sesamum Indicum L.), Verities. Int. J. Curr. Microbiol. App. Sci. 7(7): 3439-3453 (2018). doi: https://doi.org/10.20546/ijcmas.2018.707.399

[56] Ramadhas A.S., Muraleedharan C., Jayaraj S. Performance and Emission Evaluation of a Diesel Engine Fueled with Methyl Esters of Rubber Seed Oil. Renewable Energy, 30(12): 1789-1800 (2005).

[57] Candeia R.A., Silva M.C.D., Carvalho Filho J.R., Brasilino M.G.A., Bicudo T.C., Santos I.M.G., Souza A.G. Influence of Soybean Biodiesel Content on Basic Properties of Biodiesel–Diesel Blends. Fuel, 88(4): 738-743 (2009).

[58] Labeckas G., Slavinskas S., The Effect of Rapeseed Oil Methyl Ester on Direct Injection Diesel Engine Performance and Exhaust Emissions. Energy Conversion and Management, 47(13-14): 1954-1967 (2006).

[59] Thirumurugaveerakumar, Raghul, Sam Vimal Kumar Ajay R, Production and Property Analysis of Biodiesel from Cotton Seed Oil Using Natural Catalyst, International Journal of Applied Engineering Research, 13: 5672-5679 (2018).

[60] Sarin R., Sharma M., Sinharay S., Malhotra R.K. Jatropha–Palm Biodiesel Blends: An Optimum Mix for Asia. Fuel, 86(10-11): 1365-1371 (2007).

[61] Benjumea P., Agudelo J., Agudelo A. Basic Properties of Palm Oil Biodiesel–Diesel Blends. Fuel, 87(10-11): 2069-2075 (2008).

[62] Barnwal B.K., Sharma M.P. Prospects of Biodiesel Production from Vegetable Oils in India. Renewable and Sustainable Energy Reviews, 9(4): 363-378 (2005).

[63] Lafont J. J., Espitia A.A., Sodré J.R. Potential Vegetable Sources for Biodiesel Production: Cashew, Coconut and Cotton. Materials for Renewable and Sustainable Energy, 4(1): 1 (2015).

[64] Abderrabba M. Biodiesel Production from Unrefined and Refined Olive Pomace Oil: Comparative Study. Journal of Chemical and Pharmaceutical Research, 6(12): 906-915 (2014).

[65] Saydut A., Duz M.Z., Kaya C., Kafadar A.B., Hamamci C. Transesterified Sesame (Sesamum Indicum L.) Seed Oil as a Biodiesel Fuel. Bioresource Technology, 99(14): 6656-6660 (2008).

[66] Al-lwayzy S.H., Yusaf T. Diesel Engine Performance and Exhaust Gas Emissions using Microalgae Chlorella Protothecoides Biodiesel. Renewable Energy, 101: 690-701 (2017).

[67] Islam M.A., Rahman M.M., Heimann K., Nabi M.N., Ristovski Z.D., Dowell A., ... Brown R.J. Combustion Analysis of Microalgae Methyl Ester in a Common Rail Direct Injection Diesel Engine. Fuel, 143: 351-360 (2015).

[68] Nautiyal P., Subramanian K.A., Dastidar M.G. Production and Characterization of Biodiesel from Algae. Fuel Processing Technology, 120: 79-88 (2014).

[69] Haik Y., Selim M.Y., Abdulrehman T. Combustion of Algae Oil Methyl Ester in an Indirect Injection Diesel Engine. Energy, 36(3): 1827-1835 (2011).

[70] Kumar V., Muthuraj M., Palabhanvi B., Ghoshal A.K., Das D. Evaluation and Optimization of Two Stage Sequential in Situ Transesterification Process for Fatty Acid Methyl Ester Quantification from Microalgae. Renewable Energy, 68: 560-569 (2014).