Measurement of Glutamate Using Biosensor Based on Vertically Aligned Carbon Nanotubes

Document Type : Research Article


1 Institute for Nanoscience and Nanotechnology (INST), Sharif University of Technology, Tehran, I.R. IRAN

2 Thin Film and Nano-electronic Laboratory, Nano-electronic Center of Excellence, Department of Electrical and Computer Engineering, University of Tehran, Tehran, I.R. IRAN

3 Chemical & Petroleum Engineering Department, Sharif University of Technology Tehran, I.R. IRAN


A sensitive glutamate biosensor is prepared based on glutamate dehydrogenase/vertically aligned carbon nanotubes (GLDH, VACNTs). Vertically aligned carbon nanotubes were grown on a silicon substrate by Direct Current Plasma Enhanced Chemical Vapor Deposition (DC-PECVD) method. Glutamate dehydrogenase covalently attached on tip of VACNTs. The electrochemical performance of the electrode for detection of glutamate was investigated by cyclic and differential pulse voltammetry. The linear calibration curve of the concentration of glutamate versus peak current is investigated in a wide range of 0.1–500 mM. The mediator-less biosensor has a low detection limit of 57 nM and two linearranges of 0.1–20 mM with a sensitivity of 0.976 mA/mM cm2 and 20–300 mM with a sensitivity of 0.182 mA/mM.cm2. The effects of the other biological compounds on the voltammetric behavior of the prepared biosensor and its response stability are investigated. The results are demonstrated that the GLDH/VACNTs electrode even without electron mediator is a suitable basic electrode for detection of glutamate.


Main Subjects

[1] Martin S.J., Grimwood P.D., Morris R.G.M., Synaptic Plasticity and Memory: An Evaluation of the Hypothesis, Annu. Rev. Neurosci., 23, p. 649 (2000).
[2] Nedergaard M., Takano T., Hansen A., Beyond the Role of Glutamate as a Neurotransmitter, Nat. Rev. Neurosci., 3, p. 748 (2002).
[3] Meng L., Wu P., Chen G., Cai C., Sun T., Yuan Z., Low Potential Detection of Glutamate Based on the Electrocatalytic Oxidation of NADH at Thionine/ Single-Walled Carbon Nanotubes Composite Modified Electrode, Biosens. Bioelectron., 24, p. 1751 (2009).
[4] Benveniste H., Huttemeier P.C., Microdialysis: Theory and Application, Prog. Neurobiol., 35, p. 195 (1990).
[5] Hu Y., Mitchell K.M., Albahadily F.N., Michaelis E.K., Wilson G.S., Direct Measurement of Glutamate Release in the Brain Using a Dual Enzyme-Based Electrochemical Sensor, Brain Res., 659, p. 117 (1994).
[6] Gorton L., Dominguez E., Electrochemistry of NAD(P)+/NAD(P)H, in: G.S. Wilson (Ed.), "Encyclopedia of Electrochemistry (Bioelectrochemistry)", vol. 9, Wiley-VCH, Weinheim, pp. 67–143 (2002).
[7] Musameh M., Wang J., Merkoci A., Lin Y., Low-Potential Stable NADH Detection at Carbon-Nanotube-Modified Glassy Carbon Electrodes, Electrochem. Commun., 4, p. 743 (2002).
[8] Wang J., Deo R.P., Poulin P., Mangey M., Carbon Nanotube Fiber Microelectrodes, J. Am. Chem. Soc., 125, p. 14706 (2003).
[9] Schuvailo O.M., Soldatkin O.O., Lefebvre A., Cespuglio R., Soldatkin A.P.., Highly Selective Microbiosensors for in Vivo Measurement of Glucose, Lactate and Glutamate, Anal. Chim. Acta., 573, p. 110 (2006).
[10] Arumugam P.U., Chen H., Siddiqui S., Weinrich J.A.P., Jejelowo A., Li J., Meyyappan M., Wafer-Scale Fabrication of Patterned Carbon Nanofibernanoelectrode Array: Aroute for Development of Multiplexed, Ultrasensitive Disposable Biosensors, Biosens. Bioelectron, 24, p. 2818 (2009).