Studies of the Interaction of Ni (II) Complexes Bearing Planar Aromatic Ligands with Calf Thymus DNA

Document Type : Research Article


1 Islamic Azad University, Science & Research Branch, Zahedan, I.R. IRAN

2 Institute of Biochemistry and Biophysics, University of Tehran, Tehran, I.R. IRAN

3 Department of Chemistry, University of Sistan & Baluchestan, Zahedan, I.R. IRAN

4 Nanotechnology and Advanced Materials Department, Materials and Energy Research Center, Karaj, I.R. IRAN

5 Department of Biological Sciences, Kharazmi University, Tehran, I.R. IRAN


Three Nickel (II) complexes of formula [Ni(en)2](NO3)2(a), [Ni(bpy)2](NO3)2 (b) and [Ni(phen)2](NO3)2(c) (where en = ethylenediamine, bpy = 2,2 bipyridine and phen = 1,10 phenanthroline) were prepared. The structure of these complexes have been characterized by spectroscopic methods such as infrared, ultraviolet visible and nuclear magnetic resonance and conductivity measurements and elemental analysis. Interaction of these three complexes with calf- thymus DNA were carried out using ultraviolet- visible, fluorescence and jell- filterationtachniques. These complexes can denature the DNA at quite low concentrations. Complexes b and c having aromatic surface presumably intercalate in DNA. Moreover complex c can cleave the DNA into two parts and interact with both.


Main Subjects

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