Optimization of Surfactin Production by Bacillus subtilis NLIM 0110 in Shake Flask

Document Type : Research Article


1 Faculty of Chemical Engineering, Babol Noshirvani University of Technology, Babol, I.R. IRAN

2 School of Chemical Engineering, College of Engineering, University of Tehran, Tehran, I.R. IRAN


In this research, biosurfactant production (surfactin) by Bacillus subtilis NLIM 0110 has been investigated. This microorganism was isolated from agricultural area of Iran. For surfactin production, the best condition of biosurfactant in shake flask was obtained at 37°C and 250 rpm. In this condition, maximum production of biomass and biosurfactant are 4 and 1.8 g/L. The yield of biosurfactant on biomass (Yp/x), biosurfactant on sucrose (Yp/s), and the volumetric production rate (Y) for shake flask were obtained about 0.45 g/g, 0.18 g/g, and 0.03 g/L.h, respectively. For this microorganism, the production of biosurfactant was proportional to the cell growth representing biosurfactant as a growth-associated product.  The produced surfactin showed good surface activity because it is able to reduce the surface tension from 72 mN/m to 25 mN/m. Therefore, the proposed biosurfactant is appropriate candidate for industrial applications.  


Main Subjects

[1] Sen R., Biotechnology in Petroleum Recovery: The Microbial EOR, Progress in Energy and Combustion Science, 34, p. 714 (2008).
[2] راشدی ح.، جمشیدی ا.، مظاهری اسدی م.، بنکدار پور ب.، بررسی تولید رامنولیپید توسط میکروارگانیسم سودوموناس آرجینوزا جدا شده از مخازن نفتی، نشریه شیمی و مهندسی شیمی ایران، (1) 25، ص. 25 (1385)
[3] امینی ف.، صمدی ن.، هرانده م.، نقدی م.، شریفان ان.، بررسی شرایط تولید رامنولیپید حاصل از سویه های مختلف سودوموناس آرجینوزا،  نشریه علوم تغذیه و صنایع غذایی ایران، 1، ص. 33 (1388).
[4] Carrillo P.G., Mardaraz C., Pitta-Alvarez S.I., Giulietti A.M., Isolation and Selection of Biosurfactant-Producing Bacteria, World Journal of Microbiology and Biotechnology, 2, p.82 (1996).
[5] Desai J. D, Banat I. M., Microbial Production of Surfactants and Their Commercial Potential, Microbio. Mol. Biol., 61, p. 47 (1997).
[6] Sim L., Ward O., Li Z.Y., Production and Characterization of a Biosurfactant Isolated from Pseudomonas aeruginosa UW-1, J. Ind. Microbiol. Biotechnol. 19, p. 232 (1997).]
[7] Abu-Ruwaida A.S., Banat I.M., Haditirto S., Isolation of Biosurfactant Producing Bacteria, Product Characterization and Evaluation, Biotechnology, 2, p.315 (1991).
[8] Müller M.M., Hörmann B., Syldatk C.,  Hausmann R., Pseudomonas aeruginosa PAO1 as a Model for Rhamnolipid Production in Bioreactor Systems, Appl. Microbiol. Biotechnol., 87, p. 167 (2010).
[9] امانی ح.، بررسی فرایند ازدیاد برداشت میکروبی نفت با بیوسورفکتنت­ها، پایان نامه دکتری، دانشکده فنی، دانشگاه تهران، (1389).
[10] Amani H., Mehrnia M.R., Haghighi M., Sarrafzadeh M.H., Soudi M.R., Scale up and Application of Biosurfactant from Bacillus subtilis in Enhanced Oil Recovery, Applied Biochemistry and Biotechnology, 162 , p.510 (2010).
[11] Chen C.Y., Baker S.C., Darton R.C., Batch Production of Biosurfactant with Foam Fractionation, Chem. Technol. Biotechnol., 81, p. 1923 (2006).
[12] Paolo C., paola C., Method of Producing Surfactin with the Use of Mutant of Bacillus subtilis, USP 5,227,294, (1993).
[13] Veenandig N.K., Gowthaman M.K., Karanth N.G.K., Scale up Studies for the Production of Bioosurfactant in Packed Column Bioreactor, Bioprocess Engineering, 22, p. 95 (2000).
[14] Davis D.A., Lyncha H.C., Varley J., The Application of Foaming Recovery of Surfactin from B. subtilis ATCC 21332 Cultures, Enzyme Microbiol. Technol., 28, p. 346 (2001).
[15] Yeh M.S., Wei Y.H., Chang J.S., Bioreactor Design for Enhanced Carrier-assisted Surfactin Production with Bacillus subtilis, Process Biochemistry, 41, p. 1799 (2006).
[16] Joshi S., BharuchaC.,  Jha S., Yadav S., Nerurka A.,  Desai A.J.,  Biosurfactant Production Using Molasses and Whey under Thermophilic Conditions,  Bioresource Technology, 99, p. 195 (2008).
[17] Nasr S., Soudi M.R., Mehrnia M.R., Sarrafzadeh M.H.,Characterization of Novel Biosurfactant Producing Strains of Bacillus spp. Isolated from Petroleum Contaminated Soil, Iranian Journal of Microbiology, 1, p. 54 (2009).
[18] Youssef N.H., Duncan K.E., Nagle D.P., Savage K.N., Knapp R.M., McLnerney M.J., Comparison of Methods to Detect Biosurfactant Production by Diverse Microorganisms, J.of Microbiol. Meth., 56, p.339 (2004).
[19] Lin S.C., Recovery and Purification of the Lipopeptide Biosurfactant of Bacillus subtilis by Ultrafiltration, Biotechnol. Techniques, 11, P. 413 (1997).
[20] Cooper D.G., Macdonald C.R., Duff  S.F.B., Kosaric N., Enhanced production of Surfactin from Bacillus subtilis by Continuous Product Removal and Metal Cation Additions, Appl.  Environ.  Microbiol. 42, p.408 (1981).
[21] Valter P.J., Lipopeptides, An Attractive Class of Microbial Surfactants, Prog. Colloid. Polym. Sci. 72, p. 12 (1986).