Pseudomonas aeruginosa NP2 as a Model for Rhamnolipid Production in Bioreactor Systems

Document Type : Research Article

Author

Faculty of Chemical Engineering, Babol Noshirvani University of Technology, Babol, I.R. IRAN

Abstract

Rhamnolipids are among the best known biosurfactants and have been proven to be very promising in enhanced oil recovery, cosmetics, food and pharmaceutics. In this research, rhamnolipid production by Pseudomonas aeruginosa NP2 in a bioreactor has been investigated. This study shows that the Pseudomonas aeruginosa NP2 is able to produce high quantities of rhamnolipid during 2.5 L batch bioreactor cultivations with sunflower oil as sole carbon source. The results showed that the maximum rhamnolipid concentration obtained was 13.5 g/L after 80 h of cultivation. The results also showed that the rhamnolipid production started at exponential growth phase and continued during the stationary phase. Two rhamnolipids, RL1 and RL3, produced by the microorganism, were confirmed by TLC and HPLC analysis. Finally, the produced rhamnolipid showed good surface activity because it is able to reduce the surface tension from 68 mN/m to 26 mN/m. Thus, Pseudomonas aeruginosa NP2 could be an appropriate model for rhamnolipid production in pilot plant bioreactor systems.  

Keywords

Main Subjects


[1] Sen R., Biotechnology in Petroleum Recovery: The microbial EOR, Progress in Energy and Combustion Science34, p. 714 (2008).
[2] Nasr S., Soudi M.R., Mehrnia M.R., Sarrafzadeh M.H.,Characterization of Novel Biosurfactant Producing Sstrains of Bacillus spp. Isolated from Petroleum Contaminated Soil, Iranian Journal of Microbiology1, p. 54 (2009).
[3] راشدی ح.، جمشیدی ا.، مظاهری اسدی م.، بنکدار پور ب.، بررسی تولید رامنولیپید توسط میکروارگانیسم سودوموناس آرجینوزا جدا شده از مخازن نفتی، نشریه شیمی و مهندسی شیمی ایران، (1) 25، ص. 25 (1385) 
[4] امینی ف.، صمدی ن.، هرانده م.، نقدی م.، شریفان ان.، بررسی شرایط تولید رامنولیپید حاصل از سویه­های مختلف سودوموناس آرجینوزا،  نشریه علوم تغذیه و صنایع غذایی ایران، 1، ص. 33 (1388).
[5] Fiechter A., Integrated System for Biosurfactant Synthesis, Biotechnology2, p.1739 (1992).
[6] Nereus W., Daniel K.Y., Processes for the Production of Rhamnolipids, USP 7,202,063 B1. (2007).
[7] Hörmann B., Müller M.M., Syldatk C., Hausmann R., Rhamnolipid Production by Burkholderia plantarii DSM 9509, Eur. J. Lipid Sci. Technol.112, p. 674 (2010).
[8] Müller M.M., Hörmann B., Syldatk C.,  Hausmann R., Pseudomonas aeruginosa PAO1 as a Model for Rhamnolipid Production in Bbioreactor Systems, Appl. Microbiol. Biotechnol., 87, p. 167 (2010).
[9] Davis D.A., Lyncha H.C., Varley J., The Application of Foaming Recovery of Surfactin from B. subtilis ATCC 21332 Cultures, Enzyme Microbiol. Technol., 28, p. 346 (2001).
[10] Chen C.Y., Baker S.C., Darton R.C., Batch Production of Biosurfactant with Foam Fractionation, Chem. Technol. Biotechnol., 81, p. 1923 (2006).
[ا11] مانی ح.، بررسی فرایند ازدیاد برداشت میکروبی نفت با بیوسورفکتنت­ها، پایان نامه دکتری، دانشکده فنی، دانشگاه تهران، (1389).
[12] Syldatk C., Lang S., Wagner F., Wray V., Witte L., Chemical and Physical Characterization of Four Interfacial-Active Rhamnolipids from Pseudomonas Spec. DSM 2874 Grown on n-Alkanes, Z Naturforsch40, p.51 (1985).
[13] Benincasa M., Contiero J., Manresa M.A., Moraes I.O., Rhamnolipid Production by Pseudomonas aeruginosa Lbi, Journal of Food Engineering, 54, p.283 (2002).