Biosurfactant, Fermentation, Microbial enhanced oil recovery, Surface tension, Rhamnolipid

Document Type : Research Article


1 Department of Biology, Faculty of Science, Ilam University, Ilam, I.R. IRAN

2 Department of Soil Science, Faculty of Agriculture Engineering and Technology, Tehran University, Tehran, I.R. IRAN

3 Environnemental Département, Ecoles des mines De Nantes University, France


In this study, microbial enhanced oil recovery was investigated by rhamnolipid biosurfactants.  Surface tension reduction, the ability to emulsify oil, ecological acceptability, high biodegradability, stability at salinities and temperature are among the main characteristics of these biosurfactants. In this research, the ability of Pseudomonas aeruginosa NP2 to produce rhamnolipid was shown and the production of rhamnoilipd was confirmed by TLC and HPLC analysis. The rhamnolipid production started at exponential growth phase and continued during the stationary phase.During investigation of rhamnolipid production on sunflower oil as sole carbon, the results showed that the maximum rhamnolipid was reached about 0.8 g/L. Finally, the ability in recovering the oil was also demonstrated using the rhamnolipid at CMC concentration. For core flooding tests,first the brine (1% by weight) was injected at the rate of 10 mL/h until no more oil was produced in the effluent, theninjection of rhamnolipid instead of water was done. The recovery value of oil after water flooding was obtained 61.68 % of original oil in place, whereas nearly 68.56 % of original oil in place was recovered using rhamnolipd after water flooding or in other words about 6.76% enhanced oil recovery was obtained by rhamnolipid injection in core.  


Main Subjects

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