Investigation of the Zinc Extraction Possibility from Low Grade Oxide Minerals Using Heterotrophic Bacteria Pseudomonas Aeruginosa and Adaptation of Bacteria to High Concentrations of Zinc Ion

Document Type : Research Article


1 Iranian Academic Center for Education, Culture and Research (ACECR), Branch of Amirkabir, Tehran, I.R. IRAN

2 Department of Mining and Metallurgical Engineering, Amirkabir University of Technology, Tehran, I.R. IRAN


Nowadays, due to the exhaustion of high-grade mining resources and environmental considerations, the processing of mining wastes or low-grade ores to extract metals values using microorganisms has been attracted attention in industry. In this study, the ability of Pseudomonas aeruginosa (ATTC 9027) bacteria to leach zinc from low-grade zinc oxide ores of Zanjan Angoran mine has been investigated. In culture media contains glucose, this bacteria produces organic acids which dissolve zinc from its ore. Ore sample contains 14% zinc that occurred in Smithsonite (ZnCO3) mineral. Pseudomonas aeruginosa bacteria have been inoculated in culture media that contained glucose then optimum conditions have been determined. The solid/liquid ratio1/100, glucose in culture media 6% and leaching time 5 days were optimum condition and effective parameters on ore sample bioleaching and 41% of zinc content has been dissolved in these conditions. After this step using sequential cultivation technique bacteria was adapted to high concentrations of zinc ion present in this environment and the final test using adapted bacteria and in offer mentioned optimum conditions was performed and the zinc extraction was reached to 62%. Conditions have been controlled by pH measurement and zinc extraction determination at the same intervals.  


Main Subjects

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