The Synthesise of Microbial Cellulose from Local Strain and Investigating the Nanofibre Network Produced by Different Saccharide

Document Type : Research Article


1 South Tehran Branch, Islamic Azad University, Tehran, I.R. IRAN

2 Medical Sciences Faculty, Tarbiat Modares University,Tehran, I.R. IRAN


Limitation of natural resources especially cellulose reached by plant caused many researches has done for choosing on appropriate source of this material. Therefore, various methods have been evaluated for production cellulose. One of the significant methods of this biopolymer is using some different kind of bacteria which during recent decade has developed. Bacterial cellulose has produced by saccharide carbohydrate source. In this article, at first bacterial cellulose nano fibers layer were produced from three different saccharide sources (glucose, sakaroz & lactose) in static medium culture. Moisture regain, FTIR, XRD and SEM were measurement methods which applied to investigate the structure of bacterial cellulose nano fibrille and finally compared with two cellulosic fibre (cotton & viscose). The results indicated that the width of bacterial cellulose fibril nano fibre was less than 100 nanometer. The crystallinity of produced layer from three sources was less than cotton (10-15) and was more than viscose (9%-13%). The crystal structure of all produced bacterial cellulose was type 1 but the amount of cellulose was different. The result of Moisture regain showed more amounts compared to Cotton(5%) and less than regenerated cellulose and viscose (3%).


Main Subjects

[1] Retegi A., Gabilondo N., Pena C., de la Caba K., Mondragon I., Bacterial Cellulose Flms with Controlled Microstructure-Mechanical Property Relationships, Cellulose, 17, p. 661 (2010).
[2] Shezada O., Khana S., Khanb T., Kon Park J., Physicochemical and Mechanical Characterization of Bacterial Cellulose Produced with an Excellent Productivity in Static Conditions Using a Simple Fed-Batch Cultivation Strategy, Carbohydrate Polymers, 82, p. 173 (2010).
[3] Barreiro A.M., Recouvreux D.O., SPorto L.M., Rambo C.R., Hotza D., Sand Dollar Skeleton as Templates for Bacterial Cellulose Coating and Apatite Precipitation, J Mater Sci, 45, p. 5252 (2010).
[4] Cai Z., Kim J., Bacterial Cellulose/Poly(Ethylene Glycol) Composite:Characterization and First Evaluation of Biocompatibility, Cellulose, 17, p. 83 (2010).
[5] Khajavi R., Jahangirian Esfahani E., Sattari M., Crystalline Structure of Microbial Cellulose Compared with Native and Regenerated Cellulose, International Journal of Polymeric Materials, 60, p. 1178 (2011).
[6] Yang C., Tang T., Zhang S., Dai K., Gao C., Wan Y., Preparation and Characterization of Three-Dimension Nanostructured Macroporous Bacterial Cellulose/Agarose Scaffold for Tissue Engineering, J Porous Mater, 18, p. 545 (2010).
[7] Eichhorn S.J., Dufresne A., Aranguren M., Marcovich N. E., Review: Current International Research into Cellulose Nanofibres and Nanocomposites, J Mater Sci, 45, p. 1 (2010).
[8] Goelzer F.D.E., Faria-Tischer P.C.S., Vitorino J.C., Sierakowski Maria -R., Tischer C.A., Production and Characterization of Nanospheres of Bacterial Cellulose from Acetobacterxylinum from Processed Rice Bark, Materials Science and Engineering C, 29, p. 546 (2009).
[9] Meftahi A., Khajavi R., Rashidi A., Sattari M., Yazdanshenas M. E., Torabi M., The Effects of Cotton Gauze Coating with Microbial Cellulose, Cellulose, 17, p. 199 (2010).
[10] Kumar Pandey L., Saxena Ch., Dubey V., Studies on Pervaporative Characteristics of Bacterial Cellulose Membrane, Separation and Purification Technology, 42, p. 213 (2005).
[11] Kim j., Cai Z., Lee H. S., Choi G. S., Lee D. H., Jo C., Preparation and Characterization of a Bacterial Cellulose/Chitosan Composite for Potential Biomedical Application, J Polym Res, 18, p. 739 (2010).
[12] Thawatchai M., Seiichi T., Ratana R., Impregnation of Silver Nanoparticles into Bacterial Cellulose for Antimicrobial Wound Dressing, Carbohydrate Polymers, 72, p. 43 (2008) .
[13] Wojciech K. Czaja, David J. Young, Marek  Kawecki, R. Malcolm Brown, Jr. The Future Prospects of Microbial Cellulose in Biomedical Applications, Biomacromolecules, 8(1), p. 1 (2007).
[14] Klemm D., Schumann D., Udhardt U., Marsch S., Bacterial Synthesized Cellulose-Artificial Blood Vessels for Micrpsurgery, Program In Polymer Science, 26, p. 1561 (2001).
[15] Alexander S., Robert H., "Biopolymers For Medical and Pharmaceutical Application", 1st Reprint, Wiley - VCH (2005).
[16] Brown M., Johnson B., "Microbial Synthesis of Cellulose", 1st Reprint, The University of Texas at Austin, (1982).
[17] Yu X., Rajai H.A., Production of Cellulose II by Acetobacter Xylinum in the Presence of     2,6-dichlorobenzonitrile, International Journal of Biological Macromolecules, 19, p. 145(1996).
[18] Keshk S., Sameshima K., Evaluation of Different Carbon Sources for Bacterial Cellulose Pproduction, African Journal of Biotechnology, 4(6), p. 478 (2005).
[19] Surma B., Presler S., Dariusz D., Characteristics of Bacterial Cellulose Obtained from Acetobactor Xylinum Culture for Application in Papermaking, Fibres & Textiles in Eastern Europe, l16 , 4(69), p. 108 (2007).
[20] Yan Z., Chen S., Wang H., Wang B., Wang C., Cellulose Synthesized by Acetobacter Xylinum in the Presence of Multi-Walled Carbon Nanotubes, Carbohydrate Research, 343, p. 73 (2008).
[21] Phisalaphong M., Jatupaiboon N., Biosynthesis and Characterization of Bacteria Cellulose-Chitosan Film, CarboHydrate, 74, p. 482 (2008).
[22] Chen S., Zou Y., Yan Zh., Shen W., Shi S., Zhang X., Wang H., Carboxymethylated-bacterial Cellulose for Copper and Lead ion Removal, Journal of Hazrdous Materials, 161, p. 1355 (2008).
[23] Keshk S., Homogenous Reactions of Cellulose from Different Natural Sources, Carbohydrate Polymers, 74, p. 942 (2008).
[24] Golezer F., Tischer P., Vitorino J., Sierakowski M., Tischer C., Production and Characterization of Nanospheres of Bacterial Cellulose from Acetobacterxylinum from Processed Rice Bark, Materials Science and Engineering, 29 p. 546 (2008).
[25] Oh S.Y., Yoo D.I., Shin Y., Kim H.C., Kim H.Y., Chung Y.S., Park W.H., Youk J.H., Crystalline Structure Analysis of Cellulose Treated with Sodium Hydroxide and Carbon Dioxide by Means of X-Ray Diffraction and FTIR Spectroscopy, Carbohydrate Research, 340, p. 2376 (2005).
[26] Wang S., Cheng Q., Rials T.G., Lee S.H., "Cellulose Microfibril/Nanofibril and Its Nanocompsites, Tennessee Forest Products Center", University of Tennessee, pp. 303-308(2006).
[27] Li X., Chen S., Hu W., Shi S., Shen W., Zhang X., Wang H., In Situ Synthesis of CdS Nanoparticles on Bacterial Cellulose Nanofibers, Crabohydrate Polymers, 76 p. 509 (2009).
[28] Atalla R.H., Hackney J.M., Hemicelluloses as Structure Regulators in the Aggregation of Native Cellulose, Int. J. Biol. Macromol, 1, p. 109 (1993).
[29] Chung C., Lee M., Choe E.K., Characterization of Cotton Fabric Scouring by FT-IR ATR Spectroscopy, Carbohydrate Polymers, 58, p. 417 (2004).
[30] Oh S.Y., Yoo D.I., Shin Y., Sep G., FTIR Analysis of Cellulose Treated with Sodium Hydroxide and Carbon Dioxide, Carbohydrate Research, 340, p. 417 (2005).
[31] Brown E., Bacterial Cellulose/Thermoplastic Polymer Nanocomposites, Master of Science in  Chemical Engineering, Washington State University(2007).
[32] Morton W. E., and Hearle J. W. S., Physical Properties of Textile Fibers, 1 st Reprint The Textile Institute, Manchester (1986).
[33] Yan Z., Chen S., Wang H., Wang B., Jiang J., Biosynthesis of Bacterial Cellulose/Multi-Walled Carbon Nanotubes in Agitated Culture, Carbohydrate Polymers, 74, p. 1 (2008).