Document Type : Research Article
Multilayer nanometer substrates based on graphite have emerged as a new field in the design and manufacture of biocatalysts. Layered and self-assembled Nano surfaces can provide a significant accessible surface for immobilisation of drugs, biological systems and active enzymes and also can be used for detection of biomarkers. In this study, a new bioelectrode was fabricated by self-assembling a porous graphene pattern with G rich polymer (GRP). The highly hydrophobic porous graphene was functionalised with guanine (G) rich polymer to incorporate glucose oxidase (GOx). Graphene sheet dispersed in water with guanine (G) rich polymer. The electrochemical response of the bioelectrode to glucose was studied as a model for bioelectrocatalysis. The bioelectrode displayed a linear response to glucose concentration ranging from 0.2 to 2 mM, with a detection limit of 0.086 µM and 0.092 µA/µM/cm2 sensitivity. This study illustrates the potential of porous graphene for the construction of bioelectronics interfaces for the fabrication of electrochemical bio-devices.