Cation Exchange Resin Based on Polybutyl Methacrylate in Determination of Hemoglubin A1c

Document Type : Research Article


1 Department of Chemistry, Central Tehran Branch, Islamic Azad University, Tehran, I.R. IRAN

2 Iran Polymer & Petrochemical Institute, P.O. Box 14965-115 Tehran, I.R. IRAN


Determination of glycated proteins is effective in the monitoring of long-term glucose control in individuals with diabetes mellitus. Nowadays micro-column ion-exchange chromatography that could separate the proteins is frequently used. In this system, the separation was carried out on an ion-exchange resin. One of these resins based on polybutyl methacrylate is synthesized, using suspension polymerization technique. The synthesized resin was characterized by analytical methods. FT-IR spectroscopy confirmed presence of carboxylic groups in the resin. Using Scanning Electron Microscope (SEM) and BET surface analysis techniques, respectively, macroporous structure of the resin was confirmed and specific surface area was determined. Application of the resin in separation of hemoglobin A1c was examined by UV-spectrophotometer as detector. It is shown that the synthesized macropore cation exchange resin with cation exchange capacity of 3.083 mEq / (g dry resin) is applicable for Hb A1c hemoglobin separations.


Main Subjects

[1] Small H., “Ion Chromatography”. Plenum Press, New York, (1989).
[2] Haddad P.R., Jackson P.F., “Ion Chromatography”. Elsevier, Amsterdam, (1990).
[3] Walton H.F, Rocklin R.D., “Ion Exchange in Analytical Chemistry”. CRC Press, Boca Raton, (1990).
[4] Weiss J., “Ionenchromatographie”, Wiley-VCH, Weinheim, USA, p. 952 (1993).
[5] Helffrerich F., “Ion Exchange”, McGraw-Hill, New York, USA, (1962).
[6] Dasgupta P.K., Ion Chromatography: The State of the Art., Anal.Chem. 64, p. 775 A (1992).
[7] Dyer A., Hudson M.J., Williams P.A., (Editors), “Ion Exchange Processes: Advances and Applications”, Royal Society of Chemistry, Cambridge, p. 371, (1993).
[8] Dorfner K., (Ed.), “Ion Exchange", Walter de Gruyer & Co., Berlin, p. 1451 (1991).
[9] Yamamoto S., “Ion Exchange Chromatography of Proteins”, Marcel Dekker, New York, p. 401, (1988).
[10] Gooding KM, Regnier F.E., (Eds.), “HPLC of Biological Macromolecules”. Marcel Dekker, New York, p. 457 (1990).
[11] Muller W., New Ion Exchangers for the Chromatography of Biopolymers. J. Chromatog, 510, p. 133 (1990).
[12] Smith R.L., Pietrzyk D.J., Liquid Chromatographic Separation of Metal Ions on a Silica Column. Anal. Chem., 56, p. 610 (1984).
[13] Rahbar, S., Abnormal Hemoglobin in Red Cells of Diabetics. Clin. Chim. Acta, 22, p. 296 (1968).
[14] Schnek A.G., Schroeder W.A., The Relation Between the Minor Components of Whole Normal Human Adult Hemoglobin as Isolated by Chromatography and Starch Block Electrophoresis, J. Am. Chem. Soc., 83, p. 142 (1961).
[15] Rahbar S., Blumenfeld O., Ranney H.M., Studies of Unusual Hemoglobin in Patients with Diabetes Mellitus. Biochem. Biophys. Res. Commun., 36, p. 838 (1969).
[16] Trivelli L.A., Ranney H.M., Lai H.T., Hemoglobin Components in Patients with Diabetes Mellitus, N. Engi.J. Med., 284, p. 353 (1971).
[17] Kynoch P.A.M., Lehmann H., Rapid Estimation (2.5h) of Glycosylated Hemoglobin for Routine Purposes, Lancet ii, 310, p.16 (1977).
[18] Welch S.G., Boucher B.J., A Rapid Micro-Scale Method for the Measurement of Haemoglobin A1(a+b+c). Diabetologia, 14, p. 209 (1978).
[19] Chou J., Robinson C.A.Jr., Spiegel A.L., Simple Method for Estimating Glycosylated Hemoglobins, and Its Application to Evaluation of Diabetic Ppatients, Clin. Chem., 24, p. 1708 (1978).
[20] Abraham E.C., Huff T.A., Cope N.D., Wilson, J.B., Bransome, E.D., Determination of the Glycosylated Hemoglobins (Hb A1) with a New Microcolumn Procedure, Diabetes, 27, p. 931 (1978).
[21] Chithambara Thanoo B., Jayakrishnan A., Preparation of Hydrogel Beads from Crosslinked Poly(Methyl Methacrylate) Microspheres by Alkaline Hydrolysis, J. Applied Polym. Sci., 39, p. 1153 (1990).
[22] Bissé E, Abraham EC. New Less Temperature-Sensitive Microchromatographic Method for the Separation and Quantitation of Qlycosylated Hemoglobins Using a Non-Cyanide Buffer System, J. Chromatog., 344, p. 81 (1985).