Application of Headspace Liquid Phase Microextraction - Gas Chromatography for the Analysis of Trace Amounts of Isoamyl Acetate

Document Type : Research Article

Authors

Department of Chemistry, Faculty of Sciences, University of Sistan & Baluchestan, Zahedan, I.R. IRAN

Abstract

The performance of Head-Space Liquid Phase Micro Extraction (HS-LPME), coupled with capillary gas chromatography, was assessed for the extraction and determination of ultra trace amounts of isoamyl acetate in some real samples. A 2.5 µL of benzyl alcohol was used as extracting solvent and experimental parameters impacting the performance of extraction were optimized as 1250 rpm stirring rate and 15 min extraction time at 30 oC with 0.34 g.mL−1 NaCl for increasing the of ionic strength of sample solution. Under the optimized conditions, the limit of detection calculated to be 0.50 µg/L. The linear range of calibration curve for isoamyl acetate was found 5-1300 µg/L with a correlation coefficient (R2) of 0.999. Tap water and liquid soap samples were successfully analyzed using the proposed method. The relative standard deviation of water samples spiked with isoamyl acetate at 0, 200 and 400 µg/L levels was from 2.79 to 2.39%; whereas for liquid soap it was 2.74% and 2.48% for standard addition and calibration curve methods respectively. Recovery for water samples were between 101.8-103.2%; and for liquid soap sample, 103.2% and 101.8% was obtained when standard addition and normal calibration curve were employed correspondingly.

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References

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