Selectively Synthesis of Molecularly Imprinted Polymer and Their Applications for Extraction Folic Acid

Document Type : Research Note


Faculty of Chemistry, Iran University of Science and Technology, Tehran, I.R. IRAN


The purpose of this study was to synthesize the molecular imprinted polymer of folic acid for identification and rapid measurement of folic acid by fluorescence instrument.  In the synthesis of molecular imprinted polymers for increasing the strength and stability of polymer, amounts of molar ratio of functional monomer and the cross-linker should be optimum. For this purpose, the acrylamide amide, Methacrylate and folic acid were used to synthesize molecular imprinting polymer.  This material was mixed with Sol-gel solution resulting from the addition of the Tetraethyl Ortho silicate (TEOS), ethanol (EtOH), hydrochloric acid (HCl) and water. Sol-Gel polymerization causes the formation of a three-dimensional polymer network in the shortest time without the need to apply high temperature and pressure The imprinted polymer was placed within the real sample of the solution of folic acid in the DMF solvent and after the absorption of folic acid by it, the residual solution absorption was measured using fluorescence instrument. The optimum molar ratios of functional monomer and cross-linker were obtained using fluorescence instrument. SEM polymer images were evaluated before and after washing by suitable solvent and the exclusion of folic acid molecules from within the polymer network. The results showed that in the synthesis of imprinted polymers with folic acid, the optimum molar ratios of the template the functional monomer and the cross-linker were obtained 1:6:20. Using scanning electron microscope images after polymer washing, due to the exclusion of folic acid molecules from within its network, the cavities with dimensions of 140-285nm was created.


Main Subjects

[2] Beitollahi H., Ghofrani Ivari S., Torkzadeh-Mahani M., Voltammetric Determination of 6-Thioguanine and Folic Acid using a Carbon Paste Electrode Modified with ZnO-CuO Nanoplates and Modifier, Materials Science and Engineering: C 69: 128-133 (2016).
[4] Modupe O., Maurras J.B., Diosady L.L., A Spectrophotometric Method for Determining the Amount of Folic Acid in Fortified Salt, Journal of Agriculture and Food Research, 2: 100060 (2020).
[5] Farajzadeh M.A., Niazi S., Sattari Dabbagh M., Development of Dispersive Solid Phase Extraction Utilizing Folic Acid as an Efficient and Green Sorbent Followed by Dispersive Liquid–Liquid Microextraction for the Extraction of Some Plasticizers from Aqueous Samples, Journal of Separation Science, 43(23): 4314-4321 (2020).
[6] Cinková K., et al., Simple and Rapid Quantification of Folic Acid in Pharmaceutical Tablets using A Cathodically Pretreated Highly Boron-Doped Polycrystalline Diamond Electrode, Analytical Letters, 49(1): 107-121 (2016).
[10] فرجی م.، آزادنیا ا.، خسروی دارانی ک.، استخراج و اندازه‌گیری فولیک‌اسید در نان غنی شده، نشریه شیمی و مهندسی شیمی ایران، (3)32: 1 تا 11 (1392).
[11] Li C., Yang Q., Wang X., Arabi M., Peng H., Li J., ... & Chen L., Facile Approach to the Synthesis of Molecularly Imprinted Ratiometric Fluorescence Nanosensor for the Visual Detection of Folic Acid, Food chemistry, 319: 126575 (2020).
 [12] Mori K., Hirase M., Morishige T., Takano E., Sunayama H., Kitayama Y., ... & Takeuchi T., A Pretreatment‐Free, Polymer‐Based Platform Prepared by Molecular Imprinting and Post‐Imprinting Modifications for Sensing Intact Exosomes, Angewandte Chemie International Edition, 58(6): 1612-1615 (2019).
[13] Şensoy K.G., Muti M., Karagözler A.E., Highly Selective Molecularly Imprinting Polymer-based Sensor for the Electrochemical Determination of Metoxuron, Microchemical Journal, 158: 105178 (2020).
[14] Bakas I., et al. Electrochemical Impedimetric Sensor based on Molecularly Imprinted Polymers/Sol–Gel Chemistry for Methidathion Organophosphorous Insecticide Recognition, Talanta, 130: 294-298130 (2014).
[15] Faraji M., Mahmoodi-Maymand M., Dastmalchi F., Green, Fast and Simple Dispersive Liquid-Liquid Microextraction Method by using Hydrophobic Deep Eutectic Solvent for Analysis of Folic Acid in Fortified Flour Samples before Liquid Chromatography Determination, Food chemistry, 320: 126486 (2020).
[16] Sadeghi H., Shahidi S.A., Naghizadeh Raeisi S., Ghorbani-HasanSaraei A., Karimi F., Electrochemical Determination of Folic Acid in Fruit Juices Samples Using Electroanalytical Sensor Amplified with CuO/SWCNTs and 1-Butyl-2, 3-dimethylimidazolium Hexafluorophosphate, Chemical Methodologies, 4(6): 743-753 (2020).
[17] Li W., Zhang X., Miao C., Li R., Ji Y., Fluorescent Paper–based Sensor based on Carbon Dots for Detection of Folic Acid, Analytical and bioanalytical chemistry, 412: 2805-2813 (2020).