Selectively Synthesis of Molecularly Imprinted Polymer and Their Applications for Extraction Folic Acid

Document Type : Research Note




The purpose of this study was to synthesize the molecular imprinted polymer of folic acid for identification and rapid and easy measurement of folic acid by fluorescence instrument. In the synthesis of molecular imprinted polymers for increasing the strength and stability of polymer, amounts of molar ratio of functional monomer and the cross-linker should be optimum. For this purpose, the acrylamide amide, Methacrylate and folic acid were used to synthesize molecular imprinting polymer. This material was mixed with Sol-gel solution resulting from the addition of the Tetraethyl Ortho silicate (TEOS), ethanol (EtOH), hydrochloric acid (HCl) and water. Sol-Gel polymerization causes the formation of a three-dimensional polymer network in the shortest time without the need to apply high temperature and pressure The imprinted polymer was placed within the real sample of the solution of folic acid in the DMF solvent and after the absorption of folic acid by it, the residual solution absorption was measured using fluorescence instrument. The optimum molar ratios of functional monomer and cross-linker were obtained using fluorescence instrument. SEM polymer images were evaluated before and after washing by suitable solvent and the exclusion of folic acid molecules from within the polymer network. The results showed that in the synthesis of imprinted polymers with folic acid, the optimum molar ratios of the template the functional monomer and the cross-linker were obtained 1:6:20. Using scanning electron microscope images after polymer washing, due to the exclusion of folic acid molecules from within its network, the cavities with dimensions of 140-285nm was created.


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