Document Type : Research Article
Faculty of Chemical Engineering, Babol Noshirvani University of Technology, Babol, Iran
chemical engineering/biotechnology . Nushirvani University of Technology . Babol . Iran
In this research, a simple and inexpensive colorimetric detection method for dopamine was reported using laser cutting technique on microfluidic paper-based analytical devices. The effect of various factors such as reaction time between dopamine and ferric chloride, sample volume, drying time, pH of phenanthroline solution and volume ratio of ferric chloride / dopamine were investigated and optimized. The optimal values for each of the parameters were 4 min, 1.4, 2.8 and 4.2 μl, 12 min, 7.4 and 2: 1, respectively. From subsequent analyzes using Photoshop software, LOD and LOQ were calculated 0.0171 Mµ and 0.0517 Mµ, and RSD was obtained in the range of 0.12 ٪ to 17 0.17 for n = 5, which shows the good accuracy of this method. Also in this study, the potential of the proposed method as a low cost and fast colorimetric method for dopamine determination in real samples including human blood serum was shown.