Determination of Cloxacillin by Using Co3O4 Nanoparticles-Enhanced Chemiluminescence Reaction

Document Type : Research Article


Department of Chemistry, Faculty of Science, University of Maragheh, Maragheh, I.R. IRAN


In this work, the catalytic effect of Co3O4 NanoParticles (NPs) on the luminol-O2 chemiluminescence (CL) reaction in alkaline medium was presented. By using quick precipitation method, Co3O4 NPs were synthesized and then by using Scanning Electron Microscopy (SEM), X-Ray Diffraction (XRD), Transmission Electron Microscopy (TEM), Fourier Transform InfraRed (FT-IR) spectroscopy were characterized. In the following, it was indicated that cloxacillin can increase the intensity of the luminol-O2 CL reaction. Based on these findings, a new, simple, and sensitive CL method for the determination of cloxacillin was presented. The liner range, correlation coefficient, limit of detection and reproducibility (RSD%) of the method for cloxacillin were 3.0 × 10-5 - 7.0 × 10-4 mol/L, 0.99, 2.9 × 10-5 mol/L and 2.2% respectively and analysis of each sample took about 1.0 minute. In addition, this proposed method was successfully used for the determination of cloxacillin in pharmaceutical formulation.


Main Subjects

[1] Sorouraddin, M.H. and M. Iranifam, A new chemiluminescence method for determination of EDTA in ophthalmic drugs. Chem Anal (Warsaw, Pol), 52(3): 481-490 (2007).
[2] Lara, F.J., Airado-Rodriguez D., Moreno-Gonzalez D., Huertas-Perez J.F., Garcia-Campana A.M., Applications of capillary electrophoresis with chemiluminescence detection in clinical, environmental and food analysis. A review. Anal Chim Acta. , 913: 22-40 (2016).
[3] Iranifam, M., Revisiting flow-chemiluminescence techniques: pharmaceutical analysis. Luminescence,28(6): 798–820 (2013).
[4] Iranifam, M., Analytical applications of chemiluminescence methods for cancer detection and therapy. TrAC, Trends Anal. Chem., 59(0): 156-183 (2014).
[6] Iranifam, M., Analytical applications of chemiluminescence systems assisted by carbon nanostructures. TrAC, Trends Anal. Chem., 80: 387–415 (2016).
[7] Liu Z.Z., Zhao F.R., Gao S.D., Shao J.J., Chang H.Y. , The Applications of Gold Nanoparticle-Initialed Chemiluminescence in Biomedical Detection. Nanoscale Res. Lett., 11: 1-8 (2016).
[11]   Xie, J.,  Huang Y., Co3O4 nanoparticles-enhanced luminol chemiluminescence and its application in H2O2 and glucose detection. Anal Methods, 3(5): 1149-1155 (2011).
[12] Li X.H., Bai Y.F., Feng F., Zhang Z.J., The development of a novel chemiluminescent glucose sensor using hydrophilic Co3O4@SiO2 mesoporous nanoparticles. Anal Methods, 8(14): 2923-2928 (2016).
[16] Espinosa-Mansilla A., de la Pena A.M., Gomez D.G., Canada-Canada F., HPLC determination of ciprofloxacin, cloxacillin, and ibuprofen drugs in human urine samples. . J Sep Sci. , 29(13): 1969-1976 (2006).
[20] Drackova M., Navratilova P., Hadra L., Vorlova L., Hudcova L., Determination Residues of Penicillin G and Cloxacillin in Raw Cow Milk Using Fourier Transform Near Infrared Spectroscopy. Acta Vet Brno. 78(4): 685-690 (2009).
[22] Srivastava A.K., Madhavi S., Ramanujan R. , A novel method to synthesize cobalt oxide (Co3O4) nanowires from cobalt (Co) nanobowls. Phys. Status Solidi A.  207(4): 963-966 (2010).
[25] Barni F, Lewis S.W., Berti A., Miskelly G.M., Lago G. , Forensic application of the luminol reaction as a presumptive test for latent blood detection. Talanta, 72(3): 896-913 (2007).
[26] Fan Y., Shi W., Zhang X., Huang Y., Mesoporous material-based manipulation of the enzyme-like activity of CoFe2O4 nanoparticles. J. Mater. Chem. A. , 2(8): 2482-2486 (2014).
[27] Liang S.X., Zhao L.X., Zhang B.T., Lin J.M.., Experimental studies on the chemiluminescence reaction mechanism of carbonate/bicarbonate and hydrogen peroxide in the presence of cobalt(II). J. Phys. Chem. A. 112(4): 618-623 (2008).
[28] Guan G., Yang L., Mei Q., Zhang K., Zhang Z., Han M-Y., Chemiluminescence switching on peroxidase-like Fe3O4 nanoparticles for selective detection and simultaneous determination of various pesticides, Anal Chem., 84: 9492–9497 (2012).
[29] Ma L, Kang W, Xu X, Niu L, Shi H, Li S., Flow-injection chemiluminescence determination of penicillin antibiotics in drugs and human urine using luminol-Ag (III) complex system,      J. Anal. Chem. 67(3), 219–225 (2012).
[31] ارشدی، ستار ؛ دیده بان، خدیجه ؛ رستمی پایین افراکتی، معصومه، نانومخروط بور نیتریدی BNNC جایگزین شده با جایگاه فعال شبه کلروفیل: حسگری گزینش پذیر برای گاز اکسیژن، نشریه شیمی و مهندسی شیمی ایران، 32(1) 157 تا 143 (1396).
[32] قلیزاده، اعظم ؛ شاهرخیان، سعید ؛ ایرجی زاد، اعظم ؛ مهاجرزاده، شمس الدین ؛ وثوقی، منوچهر ، اندازه‌گیری گلوتامات با استفاده از حسگر زیستی بر پایه نانولوله‌های کربنی عمودی ، نشریه شیمی و مهندسی شیمی ایران، 32(4)33 تا 36 (1392).