Manufacturing of Cubic Nanocrystallites of Silver by Nanophytosynthesis Method and Their Application in Enhance of Tetracycline Antibiotic Activities

Document Type : Research Article


1 Dep. Chemistry, Ardabil Branch, I.A. University, Ardabil, Iran

2 Department of Chemistry, Khalkhal Branch, Islamic Azad University, Khalkhal, I.R. IRAN


In this study, cubic nanocrystallites of silver nanoparticles were synthesized by the nano photosynthesis method with the utilization of the secondary metabolite of black hawthorn (Crataegus melanocarpa) leaf methanolic extract. The structure and optical properties of the obtained nanoparticles were investigated by Transmission Electron Microscopy (TEM), Scanning Electron Microscopy (SEM), X-Ray Diffractometry (XRD), Fourier Transform InfraRed (FT-IR), and UltraViolet-Visible (UV/Vis) spectroscopy. Antibacterial effect of produced silver nanocrystallites as separately and in combined with tetracycline against four Gram-positive and Gram-negative bacterial species were studied according to the disk diffusion method. The biological study showed that these silver nanoparticles obtained by the nano photosynthesis method have good affectivity against the growth of bacteria and enhances the antibacterial effect of tetracycline as an antibiotic. Increasing the inhibition of bacterial growth by these nanocrystals was attributed to their cubic structure.  Also, the nano photosynthesis method for nanoparticle preparation is an economical, fast, eco-friendly, and green synthesis method and provides optimal conditions for nanoparticle production.


Main Subjects

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