Study the Effective Factors on Aqueous Two-Phase Systems Containing PEG4000 and Phosphate Salt in Extraction of Malic Acid

Document Type : Research Article


Department of Chemical Engineering, University of Guilan, Rasht, I.R. IRAN


Novel separation procedures that are essential for the purification of biomolecules in biotechnology industries, are developing. Hence, these methods should be rapid, selective, and easy to scale up. The aqueous two-phase system is an efficient method for the purification of materials. The purpose of the present work was to investigate the various parameters such as temperature, pH, salt concentration, and salt type in the polymer/salt system. In this research, purification of malic acid in the aqueous two-phase system which is containing polyethylene glycol 4000 g/mol and three variant salt (dipotassium hydrogen phosphate, diammonium hydrogen phosphate, and disodium hydrogen phosphate) were studied. According to the results, the optimum D and Y%  for extraction of malic acid was identified 0.85 and 80%, under these conditions, including 25% (w/w) disodium hydrogen phosphate at T=25°C and pH=5, respectively.


Main Subjects

[1] Malmary G., Vezier A., Robert A., Molinier J., Mourgues J., Conte T.,  Recovery of Tartaric and Malic Acids from Dilute Aqueous Effluents by Solvent Extraction Technique, Journal of Chemical Technology & Biotechnology: International Research in Process, Environmental and Clean Technology,60(1): 67-71 (1994).
[2] Gao Q., Pan C., Liu F., Lu F., Wang D., Zhang J., Zhu Y., Adsorption Characteristics of Malic Acid from Aqueous Solutions by Weakly Basic Ion-Exchange Chromatography, Journal of Chromatography A, 1251: 148-153 (2012).
[3] Gök A., Gök M., Aşçı Y., Lalikoglu M., Equilibrium, Kinetics and Thermodynamic Studies for Separation of Malic Acid on Layered Double Hydroxide (LDH), Fluid Phase Equilibria, 372: 15-20 (2014).
[4] Gilani H.G., Samper K.G., Haghi R.K., "Advanced Process Control and Simulation for Chemical Engineers", Apple Academic Press (2016).
[5] Treybal R.E., "Mass Transfer Operations". Mc Graw-Hill, New York, (1980).
[6] Diamond A., Hsu J., "Aqueous Two-Phase Systems for Biomolecule Separation, in Bioseparation", Springer, p. 89-135 (1992).
[7] Dobry A., Boyer‐Kawenoki F., Phase Separation in Polymer Solution, Journal of Polymer Science, 2(1): 90-100 (1947).
[9] Albertsson P.-Å., Cajarville A., Brooks D., Tjerneld F.,  Partition of Proteins in Aqueous Polymer Two-Phase Systems and the Effect of Molecular Weight of the Polymer. Biochimica Et Biophysica Acta (BBA)-General Subjects, 926(1): 87-93 (1987).
[10] Khayati G., Gilani H.G., Safari Keyvani Z., Extraction of Cu(II) Ions from Aqueous Media Using PEG/Sulphate Salt Aqueous Two-Phase System, Separation Science and Technology, 51(4): 601-608 (2016).
[12] Khayati G., Optimization of Propionic Acid Extraction by Aqueous Two-Phase System Using Response Surface Methodology, Chemical Engineering Communications, 200(5): 667-677 (2013).
[13] Nandini K., Rastogi N., Liquid-Liquid Extraction of Lipase Using Aqueous Two-Phase System, Food and Bioprocess Technology, 4(2): 295-303 (2011).
[14] Wongmongkol N., Prichanont S., Partition of Alkaline Protease in Aqueous Two-Phase Systems of Polyethylene Glycol 1000 and Potassium Phosphate, Korean Journal of Chemical Engineering, 23(1): 71-76 (2006).
[15] Kordel M., Hofmann B., Schomburg D., Schmid R., Extracellular Lipase of Pseudomonas Sp. Strain ATCC 21808: Purification, Characterization, Crystallization, and Preliminary X-Ray Diffraction Data, Journal of Bacteriology, 173(15): 4836-4841 (1991).
[16] قنادزاده گیلانی، ح.؛ جنگجوی شالدهی، ط.؛ بررسی پارامتر های مؤثر بر استخراج والریک اسید به کمک سامانه­های دوفازی ابی، نشریه شیمی و مهندسی شیمی ایران، (3)38: 155 تا 162 (1399).
[17] قنادزاده گیلانی، ح.؛ جنگجوی شالدهی، ط.؛ معصومی، ح.؛ بررسی پارامترهای مؤثر بر استخراج آسکوربیک اسید
به کمک نمک‌های سولفات و پلی اتیلن گلیکول در سامانه‌های دوفازی آبی
، نشریه شیمی و مهندسی شیمی ایران،  (3)39: 163 تا 170 (1399).
[18] Marcus Y., Thermodynamics of Solvation of Ions. Part 5.—Gibbs Free Energy of Hydration at 298.15 K, Journal of the Chemical Society, Faraday Transactions, 87(18): 2995-2999 (1991).
[19] Pirdashti M., Movagharnejad K., Mobalegholeslam P., Curteanu S., Leon F., Phase Equilibrium and Physical Properties of Aqueous Mixtures of Poly (Vinyl Pyrrolidone) with Trisodium Citrate, Obtained Experimentally and by Simulation, Journal of Molecular Liquids, 223: 903-920 (2016).